1. Forward and reverse genetic approaches in Drosophila.
Forward and reverse genetic approaches in Drosophila. (A) Forward genetics uncovers the genetic basis of phenotype. Mutagenesis by any means (e.g. X-rays, chemicals or transposons; indicated by a lightning bolt) is used to generate mutant flies with aberrant phenotypes (indicated by the red fly), which are used as a starting point for gene discovery. Reverse genetics refers to the discovery of gene function through the targeted disruption of genes (here indicated by an asterisk showing a mutation in a gene sequence) and the analysis of the resulting phenotype(s). (B) Both forward and reverse genetic strategies are useful for the creation of animal models of disease that can be used as platforms to test hypotheses, perform modifier screens and identify new therapeutics. (A,B) In both panels, wild-type flies are shown in brown, mutant flies in red.
Matthew J. Moulton, and Anthea Letsou Dis. Model. Mech. 2016;9:
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