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Simultaneous cytofluorometric measurement of phagocytosis, burst production and killing of human phagocytes using Candida albicans and Staphylococcus aureus as target organisms H.R. Salih, L. Husfeld, D. Adam Clinical Microbiology and Infection Volume 6, Issue 5, Pages (May 2000) DOI: /j x Copyright © 2000 European Society of Clinical Infectious Diseases Terms and Conditions
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Figure 1 Analysis of phagocytosis; the four top diagrams represent the results at the beginning and the bottom panels show the results after incubation. In dot plot A the PMNs are selected in a bitmap; in histogram B the percentage of the previously selected cells with an additional green fluorescence (phagocytosing PMNs) along the kinetics is detected. The target organisms are selected in a bitmap in dot plot C; in histogram D the percentage of these cells with additional orange fluorescence (ingested microorganisms) is detected. Clinical Microbiology and Infection 2000 6, DOI: ( /j x) Copyright © 2000 European Society of Clinical Infectious Diseases Terms and Conditions
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Figure 2 Analysis of the oxidative burst; the top panels represent the results at the beginning, and the bottom panels show the results after incubation. In dot plot A, the microorganisms are selected in a bitmap. Oxidative compounds create the fluorescent ethidium bromide, which is scored in histogram B. The measured events represent the PMNs displaying an oxidative burst after phagocytosis. Clinical Microbiology and Infection 2000 6, DOI: ( /j x) Copyright © 2000 European Society of Clinical Infectious Diseases Terms and Conditions
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Figure 3 The kinetics of phagocytosis, burst production and killing using (A) Candida albicans and (B) Staphylococcus aureus; percentages of phagocytosing PMNs, ingested microorganisms, oxidative burst-producing PMNs and dead microorganisms. Data of one of 25 experiments with similar results are shown. Clinical Microbiology and Infection 2000 6, DOI: ( /j x) Copyright © 2000 European Society of Clinical Infectious Diseases Terms and Conditions
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