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Soybean (Glycine max) allergy in Europe: Gly m 5 (β-conglycinin) and Gly m 6 (glycinin) are potential diagnostic markers for severe allergic reactions to soy Thomas Holzhauser, PhD, Olga Wackermann, MSc, Barbara K. Ballmer-Weber, MD, Carsten Bindslev-Jensen, MD, Joseph Scibilia, MD, Lorenza Perono-Garoffo, PhD, Shigeru Utsumi, PhD, Lars K. Poulsen, PhD, Stefan Vieths, PhD Journal of Allergy and Clinical Immunology Volume 123, Issue 2, Pages e4 (February 2009) DOI: /j.jaci Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions
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Fig 1 IgE-immunoblot analysis of a soybean extract. All lanes except for 28∗ and P were detected with peroxidase mediated chemiluminescence (lane C: buffer control; lane N: nonatopic control serum pool [n = 3]; lanes 1, 3, 14, 18, 21, 30, and 28: sera from subjects with soybean allergy summarized in Table I; lane 28∗: patient 28 detected with phosphatase and precipitating dye; lane P: silver-stained total soybean protein). Journal of Allergy and Clinical Immunology , e4DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions
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Purity testing of the individual Gly m 5 (β-conglycinin) subunits β (Gly m 5.03), α (Gly m 5.01), and α′ (Gly m 5.02) from natural soybean source in SDS-PAGE and silver staining (lanes β, α, and α′), and verification of the IgE reactivity of all Gly m 5 subunits by immunoblot analysis with selected soy allergic sera (lane C: buffer control; N: nonatopic control serum pool [n = 3]; lanes 5, 14, 18, 19, and 20: subjects with soybean allergy). Journal of Allergy and Clinical Immunology , e4DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions
![Purity testing of the individual Gly m 5 (β-conglycinin) subunits β (Gly m 5.03), α (Gly m 5.01), and α′ (Gly m 5.02) from natural soybean source in SDS-PAGE and silver staining (lanes β, α, and α′), and verification of the IgE reactivity of all Gly m 5 subunits by immunoblot analysis with selected soy allergic sera (lane C: buffer control; N: nonatopic control serum pool [n = 3]; lanes 5, 14, 18, 19, and 20: subjects with soybean allergy).](http://slideplayer.com./slide/17135482/99/images/3/Purity+testing+of+the+individual+Gly+m+5+%28%CE%B2-conglycinin%29+subunits+%CE%B2+%28Gly+m+5.03%29%2C+%CE%B1+%28Gly+m+5.01%29%2C+and+%CE%B1%E2%80%B2+%28Gly+m+5.02%29+from+natural+soybean+source+in+SDS-PAGE+and+silver+staining+%28lanes+%CE%B2%2C+%CE%B1%2C+and+%CE%B1%E2%80%B2%29%2C+and+verification+of+the+IgE+reactivity+of+all+Gly+m+5+subunits+by+immunoblot+analysis+with+selected+soy+allergic+sera+%28lane+C%3A+buffer+control%3B+N%3A+nonatopic+control+serum+pool+%5Bn+%3D+3%5D%3B+lanes+5%2C+14%2C+18%2C+19%2C+and+20%3A+subjects+with+soybean+allergy%29..jpg "Journal of Allergy and Clinical Immunology , e4DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions.")
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A, Individual subunits of Gly m 6 by total protein staining of Western blots with Ponceau S (lanes: G1, Gly m 6.01, G2, Gly m 6.02; G3, Gly m 6.03; G4, Gly m 6.04; G5, Gly m 6.05) and verification of IgE-reactivity of all Gly m 6 subunits (double lane) with patient 28 (N: nonatopic serum pool). B, IgE immunoblot analysis of equal amounts (wt/wt) of the Gly m 6 subunits with selected patients' sera (lane C: buffer control; N: nonatopic control serum pool [n = 3]; lanes 1, 12, 14, 19, and 21: subjects with soybean allergy). The arrows indicate the position of the 5 proglycinin subunits according to molecular weight. Journal of Allergy and Clinical Immunology , e4DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions
![A, Individual subunits of Gly m 6 by total protein staining of Western blots with Ponceau S (lanes: G1, Gly m 6.01, G2, Gly m 6.02; G3, Gly m 6.03; G4, Gly m 6.04; G5, Gly m 6.05) and verification of IgE-reactivity of all Gly m 6 subunits (double lane) with patient 28 (N: nonatopic serum pool). B, IgE immunoblot analysis of equal amounts (wt/wt) of the Gly m 6 subunits with selected patients sera (lane C: buffer control; N: nonatopic control serum pool [n = 3]; lanes 1, 12, 14, 19, and 21: subjects with soybean allergy). The arrows indicate the position of the 5 proglycinin subunits according to molecular weight.](http://slideplayer.com./slide/17135482/99/images/4/A%2C+Individual+subunits+of+Gly+m+6+by+total+protein+staining+of+Western+blots+with+Ponceau+S+%28lanes%3A+G1%2C+Gly+m+6.01%2C+G2%2C+Gly+m+6.02%3B+G3%2C+Gly+m+6.03%3B+G4%2C+Gly+m+6.04%3B+G5%2C+Gly+m+6.05%29+and+verification+of+IgE-reactivity+of+all+Gly+m+6+subunits+%28double+lane%29+with+patient+28+%28N%3A+nonatopic+serum+pool%29.+B%2C+IgE+immunoblot+analysis+of+equal+amounts+%28wt%2Fwt%29+of+the+Gly+m+6+subunits+with+selected+patients+sera+%28lane+C%3A+buffer+control%3B+N%3A+nonatopic+control+serum+pool+%5Bn+%3D+3%5D%3B+lanes+1%2C+12%2C+14%2C+19%2C+and+21%3A+subjects+with+soybean+allergy%29.+The+arrows+indicate+the+position+of+the+5+proglycinin+subunits+according+to+molecular+weight..jpg "Journal of Allergy and Clinical Immunology , e4DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions.")
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