1. Fig. 3. Coilin levels correlate with altered nascent U2 snRNA, hTR and rRNA levels.RNA isolated from HeLa or WI-38 cells following RNAi targeting coilin was analyzed by qRT-PCR using primers specific to U2 snRNA, telomerase RNA and rRNA.
Coilin levels correlate with altered nascent U2 snRNA, hTR and rRNA levels.RNA isolated from HeLa or WI-38 cells following RNAi targeting coilin was analyzed by qRT-PCR using primers specific to U2 snRNA, telomerase RNA and rRNA. (A) Histogram of relative 47/45S precursor rRNA to 5.8S rRNA following 48-hour control or coilin knockdown in HeLa cells resulting in 87% reduction of coilin mRNA. (B) Histogram of the fold change of specific RNA transcripts following 48-hour coilin knockdown in WI-38 cells resulting in an average 96% reduction of coilin mRNA; *P<0.05 relative to control knockdown; #P<0.05 between data pairs. (C) Histogram of coilin expression, relative to GAPDH, in HeLa and WI-38 cells treated with control siRNA for 48 hours; *P<0.05 relative to HeLa. Error bars represent 1 s.d. of 3 experimental repeats (2 for WI-38 hTR) and 3 technical repeats within each experiment. All message levels were normalized to GAPDH prior to comparison with either control knockdown or between cell lines.
Hanna J. Broome et al. Biology Open 2013;2:
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