1. Endobronchial transfection of naked TGF-β1 cDNA attenuates acute lung rejection 
Franco D’Ovidio, MD, Motoki Yano, MD, Jon H Ritter, MD, Thalachallour Mohanakumar, PhD, G.Alexander Patterson, MD 
The Annals of Thoracic Surgery 
Volume 68, Issue 3, Pages (September 1999)
DOI: /S (99)

2. Fig 1
CAT protein expression in lungs after gene transfer of naked plasmid pCF1-CAT or liposome-plasmid complex GL67:pCF1-CAT performed in the non-transplant and in the transplant setting. pCF1-CAT versus GL67:pCF1-CAT within the non-transplant and within the transplant setting, respectively, p = 0.03 and p = 0.5; pCF1-CAT non-transplant versus pCF1-CAT transplant, p = 0.01; GL67:pCF1-CAT non-transplant versus GL67:pCF1-CAT transplant, p = 0.1. Error bars represent SD.
The Annals of Thoracic Surgery  , DOI: ( /S (99) )

3. Fig 2
Arterial oxygenation after mono-ventilation of the allograft at the time of sacrifice on the 5th postoperative day, when acute rejection has occurred. Significant differences were: saline control group versus pMP6A-TGF-β1 sense and versus GL67:pMP6A-TGF-β1 sense, respectively; p = and p = 0.006; pMP6A-TGF-β1 sense versus GL67:pMP6A-TGF-β1 sense and versus pMP6A-TGF-β1 antisense, respectively, p = 0.05 and p = 0.01.
The Annals of Thoracic Surgery  , DOI: ( /S (99) )

4. Fig 3
Vascular rejection score of allografts transfected with pMP6A-TGF-β1 sense, GL67:pMP6A-TGF-β1, pMP6A-TGF-β1 antisense, and in those that received saline solution. The dots show the score for each sample. When the rejection score was in between values, a 0.5 score was added to lower value.
The Annals of Thoracic Surgery  , DOI: ( /S (99) )

5. Fig 4
RT-PCR of the murine derived TGF-β1 within the rat lung parenchyma. The amplified DNA sequences are assessed by 2.5% agarose gel electrophoresis. Lane 1 shows the 100 base pair ladder. Lanes 2, 4, and 6, show the β-actin RT-PCR control (138 bp). RT-PCR for murine TGF-β1 is shown in lanes 3, 5, 7, and 8 (236 bp). Lane 3 shows the results of the RT-PCR within non-transfected rat lung. Lane 5 shows RT-PCR for murine TGF-β1 within lungs transfected with the sense murine TGF-β1 either. Lane 7 shows RT-PCR for murine TGF-β1 within mouse lung. Lane 8 shows the PCR for murine TGF-β1 performed using the sense plasmid pMP6A-TGF-β1 encoding the murine derived TGF-β1.
The Annals of Thoracic Surgery  , DOI: ( /S (99) )