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Autophagy is upregulated in ovarian endometriosis: a possible interplay with p53 and heme oxygenase-1  Giulia Allavena, M.S., Patrizia Carrarelli, Ph.D.,

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Presentation on theme: "Autophagy is upregulated in ovarian endometriosis: a possible interplay with p53 and heme oxygenase-1  Giulia Allavena, M.S., Patrizia Carrarelli, Ph.D.,"— Presentation transcript:

1 Autophagy is upregulated in ovarian endometriosis: a possible interplay with p53 and heme oxygenase-1  Giulia Allavena, M.S., Patrizia Carrarelli, Ph.D., Barbara Del Bello, Ph.D., Stefano Luisi, M.D., Ph.D., Felice Petraglia, M.D., Emilia Maellaro, Ph.D.  Fertility and Sterility  Volume 103, Issue 5, Pages e1 (May 2015) DOI: /j.fertnstert Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

2 Figure 1 Biochemical markers of autophagy indicate that autophagy is upregulated in OMA. Densitometric quantification of LC3-II protein (A), ratio LC3-II/LC3-I (B), and SQSTM1 (p62) protein (C) evaluated by Western blot. Results of protein levels in OMA and eutopic endometria of affected women (EEOMA) are reported as fold-increase over normal endometria from healthy women (NE) (mean value set to 1). (D, E) Representative Western blots. ***P<.001, OMA vs. NE or EEOMA. Fertility and Sterility  , e1DOI: ( /j.fertnstert ) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

3 Figure 2 Autophagy-related genes are upregulated in OMA. Messenger RNA levels of ATG14 (A), BECN1 (Beclin-1) (B), ATG7 (C), and LC3B (LC3) (D). Gene expression values of EEOMA and OMA samples, measured by quantitative real-time PCR, are expressed as fold-change over NE samples. *P<.05 and *** P<.001, OMA vs. NE or EEOMA. Fertility and Sterility  , e1DOI: ( /j.fertnstert ) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

4 Figure 3 Beclin-1 and Atg7 proteins decrease in OMA. Densitometric quantification of Beclin-1 (A) and Atg7 (B) proteins evaluated by Western blot. Results of protein levels in EEOMA and OMA samples are reported as fold-increase over NE samples (mean value set to 1). (C, D) Representative Western blots. *P<.05, OMA vs. NE and EEOMA, or EEOMA vs. NE. Fertility and Sterility  , e1DOI: ( /j.fertnstert ) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

5 Figure 4 p53 protein is decreased and HO-1 is induced in OMA. Densitometric quantification of p53 (A), Bcl-2 (B), and HO-1 (C) proteins evaluated by Western blot. Results of protein levels in EEOMA and OMA samples are reported as fold-increase over NE samples (mean value set to 1). (D–F) Representative Western blots. **P<.01 and ***P<.001, OMA vs. NE or EEOMA. Fertility and Sterility  , e1DOI: ( /j.fertnstert ) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions


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