1. Uncaging responses in spines that are below the optical measurement threshold: clear optical bAP measurements serve as positive controls and for signal calibration.
Uncaging responses in spines that are below the optical measurement threshold: clear optical bAP measurements serve as positive controls and for signal calibration. A–C, Same as Figure 1 on a different dendrite with target spine 52 μm from soma. A clear soEPSP is seen in B (0.5 mV peak), but no clear optical spEPSP is seen in B (top; no fit is made), whereas at the same time bAPs are clearly measureable optically in C (top; serves as positive control). Averages of N = 10 sweeps APs, 18.3% peak. Average of N = 10 sweeps uncaging and control, σnoise = 0.3%.
Corey D. Acker et al. eNeuro 2016;3:ENEURO
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