1. Effects of LMHFV and oestrogen (E2) supplementation on bone healing in OVX mice 21 days after osteotomy.
Effects of LMHFV and oestrogen (E2) supplementation on bone healing in OVX mice 21 days after osteotomy. The dotted lines indicate the values of aged-matched non-OVX mice. E2 supplementation of OVX mice significantly improved fracture healing. Additional LMHFV did not further increase bone healing. Physiological values of aged-matched non-OVX mice were not achieved. (A) Flexural rigidity (EI) of the fracture callus after 21 days. (B) Total callus volume (TV) as assessed by μCT. (C) Bone volume/total volume (BV/TV) assessed using μCT. (D) Callus composition on day 10 assessed by histomorphometry, given as a percentage of the total callus area. Bone, TOT; cartilage, Cg; and fibrous tissue, FT. (E) Callus composition on day 21 as assessed by histomorphometry. (F) Percentage of mice with bony bridging of the fracture gap. (G–I) Representative histological sections of fractured femurs 21 days after osteotomy stained using Giemsa. (G) non-vibrated OVX mouse, (H) non-vibrated OVX mouse with E2 supplementation, (I) vibrated OVX mouse with E2 supplementation. Scale bars: 500 μm. *P<0.05. n=8.
Esther Wehrle et al. Dis. Model. Mech. 2015;8:93-104
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