1. Volume 42, Issue 4, Pages 541-547 (April 2005)
Evidence for Galα(1–3)Gal expression on primary porcine hepatocytes: implications for bioartificial liver systems 
Maarten-Paul van de Kerkhove, Menno R. Germans, Tanja Deurholt, Ruurdtje Hoekstra, David H. Joziasse, Albert C.W.A. van Wijk, Thomas M. van Gulik, Robert A.F.M. Chamuleau, Anja Roos 
Journal of Hepatology 
Volume 42, Issue 4, Pages (April 2005)
DOI: /j.jhep
Copyright © 2005 European Association for the Study of the Liver Terms and Conditions

2. Fig. 1
The AMC-BAL bioreactor. (A) bioreactor; (B) cross section through bioreactor; (C) electron microscopic picture presenting porcine hepatocytes attached to polyester matrix fibers inside the bioreactor and the extra-capillary space through which the plasma flows.
Journal of Hepatology  , DOI: ( /j.jhep )
Copyright © 2005 European Association for the Study of the Liver Terms and Conditions

3. Fig. 2
Real time RT-PCR results. Quantitative ggta-1 mRNA levels normalised for gapdh mRNA levels and expressed as percentage of the initial cell isolate; Cell 1 and 2 are data from two different hepatocyte isolations; BAL 1, 3 and 7 represents data from a BAL cultured for one, three and seven days, respectively. BAL 1 and 3 were loaded with the same hepatocytes as cell 1, BAL 7 was loaded with the same hepatocytes as cell 2.
Journal of Hepatology  , DOI: ( /j.jhep )
Copyright © 2005 European Association for the Study of the Liver Terms and Conditions

4. Fig. 3
Immunohistochemistry of liver tissue. Representative figures are shown of double staining with anti-CPS-CY3 and GSI-B4-FITC; (B) staining with GSI-B4-FITC alone; (C) staining with anti-CPS-CY3 alone; (D) double staining with anti-CPS-CY3 and IgG anti-Galα(1–3)Gal; (E) staining with IgG anti-Galα(1–3)Gal alone; (F) staining with anti-CPS-CY3 alone.
Journal of Hepatology  , DOI: ( /j.jhep )
Copyright © 2005 European Association for the Study of the Liver Terms and Conditions

5. Fig. 4
Immunocytochemistry. Representative figures are shown of (A) porcine hepatocytes double stained with anti-CPS-CY3 and GSI-B4-FITC; (B) porcine hepatocytes double stained with anti-CPS-CY3 and anti-Galα(1–3)Gal IgG; (C) PK-15 cells stained with GSI-B4-FITC. Both cell types were cultured for 4h.
Journal of Hepatology  , DOI: ( /j.jhep )
Copyright © 2005 European Association for the Study of the Liver Terms and Conditions

6. Fig. 5
FACS analysis. Representative figures are shown of (A) scatter plot of hepatocyte isolate and gating of subpopulations; (B) histogram of PK-15 cells, untreated cells in grey and GSI-B4-FITC incubated cells in white; (C) histogram of liver cell isolate, untreated cells in grey and GSI-B4-FITC incubated cells in white; (D) histogram of liver cell isolate, GSI-B4-FITC incubated cells in grey and Galα(1–3)Gal-PAA saturated GSI-B4-FITC incubated cells in white. MFI=mean fluorescence intensity.
Journal of Hepatology  , DOI: ( /j.jhep )
Copyright © 2005 European Association for the Study of the Liver Terms and Conditions

7. Fig. 6
XNAB IgG and IgM concentration (arbitrary unitis/ml) measured in plasma of six patients measured directly before and immediately after BAL treatment. Patient 1=●, 2=□, 3=○, 4=▪, 5=▵, 6=▴. Paired t-test P<0.05 (both for IgG and IgM).
Journal of Hepatology  , DOI: ( /j.jhep )
Copyright © 2005 European Association for the Study of the Liver Terms and Conditions