1. Optimal polar bodies angle for higher subsequent embryo viability: a pilot study 
Qiong Zhang, M.D., Xiaoyue M. Guo, B.A., Yanping Li, M.D. 
Fertility and Sterility 
Volume 105, Issue 3, Pages e2 (March 2016)
DOI: /j.fertnstert
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
The schematic diagram of polar body (PB) angle measurement, subsequent embryo quality and implantation outcome. Lines were drawn to connect the oocyte geometric center of the zygote with the two PBs. PB angle (α) was subtended by the two lines. The PB angles of the three zygotes are (A) 16.32°, (B) 38.13°, and (C) °, respectively from left to right. Their subsequent embryos quality were all grade A on day 3. After transfer, only the leftmost embryo implanted successfully.
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Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
The flow of the study. A total of 116 patients were recruited. The polar body (PB) angles were measured in 454 two-pronucleated (2PN) zygotes. On day 3, 164 embryos were transferred into the uterus. Ultimately, all-or-none implantation took place in 129 embryos.
Fertility and Sterility  , e2DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
Receiver operating characteristic (ROC) curve of polar body angle for subsequent embryo implantation. The area under the ROC curve is (95% confidence interval 0.674–0.838) and the cutoff value is 24.25°. The sensitivity is 64.8% and the specificity 73.2%.
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Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

5. Supplemental Figure 1
The positive skew distribution of polar body (PB) angles. The PB angles showed a positive skew distribution with range from 10.11° to °. The median is 26.73° and the skew coefficient is
Fertility and Sterility  , e2DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions