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Fig. 5. The bulk of Cep135 localizes distantly from Sas-6 and STIL
Fig. 5. The bulk of Cep135 localizes distantly from Sas-6 and STIL.U2OS cells were stained with the indicated antibodies. The bulk of Cep135 localizes distantly from Sas-6 and STIL.U2OS cells were stained with the indicated antibodies. Representative 3D-SIM and wide-field (WF) images are shown. Scale bars 0.5 µm or 1 µm (overview image). (A) Localization of Sas-6, Cep135 and STIL in cells in S/G2 phase (before centrosome separation, upper panel) or late G2/M phase (after centrosome separation, lower panel). Wide-field images of the red (Cep135) and far-red (CP110) channels of the same centrosome were acquired to determine the orientation of centrioles (schematics illustrate centriole orientations and overview panel shows centrosome separation). (B) Brightness/contrast-enhanced image to indicate co-localization of weak Cep135 signal with Sas-6 (arrowheads). Katharina F. Sonnen et al. Biology Open 2012;1: © Published by The Company of Biologists Ltd
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