1. Isolates from the NZ97/192 household interact differently with host respiratory epithelial 16HBE cells.
Isolates from the NZ97/192 household interact differently with host respiratory epithelial 16HBE cells. For each assay, all isolates were tested together and in biological replicates. Each assay was performed in triplicate at least twice. (A) Viable bacteria associated with the 16HBE cells (adhered and intracellular), expressed as a percentage of the initial inoculant. Horizontal bars indicate means, while error bars indicate standard deviations. (B) Viable bacteria present inside 16HBE cells after gentamicin treatment, expressed as a percentage of the initial inoculant. Horizontal bars indicate means, while error bars indicate standard deviations. (C) Concentration of TNF-α in the culture media of 16HBE cells and bacterial cocultures (picograms per milliliter, n = 6). The average value is plotted for each isolate, and error bars indicate standard deviations.
Xiaoyun Ren et al. mSystems 2017; doi: /mSystems