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Fractional analysis of sequential induced sputum samples during sputum induction: Evidence that different lung compartments are sampled at different time points Neil H. Gershman, MD, Hong Liu, MD, Hofer H. Wong, BS, Jane T. Liu, MS, John V. Fahy, MD Journal of Allergy and Clinical Immunology Volume 104, Issue 2, Pages (August 1999) DOI: /S (99)70374-X Copyright © 1999 Mosby, Inc. Terms and Conditions
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Fig. 1 Total cell count, squamous cell percentage, and volume of induced sputum samples collected individually at five 4-minute intervals during a 20-minute sputum induction. None of the changes in these parameters were statistically significant. Journal of Allergy and Clinical Immunology , DOI: ( /S (99)70374-X) Copyright © 1999 Mosby, Inc. Terms and Conditions
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Fig. 2 Changes in cell differential and numbers of nonsquamous cells in induced sputum samples collected individually at five 4-minute intervals during a 20-minute sputum induction. The 5 panels in the column on the left show the data for eosinophil, neutrophil, macrophage, epithelial cell, and lymphocyte percentages, and the 5 panels on the right show the cell numbers of these cells. *Significantly different from the 0- to 4-minute interval (P < .05). Journal of Allergy and Clinical Immunology , DOI: ( /S (99)70374-X) Copyright © 1999 Mosby, Inc. Terms and Conditions
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Fig. 3 Changes in the levels of ECP and fibrinogen in induced sputum samples collected individually at five 4-minute intervals during a 20-minute sputum induction. *Significantly different from the 0- to 4-minute interval (P < .05). Journal of Allergy and Clinical Immunology , DOI: ( /S (99)70374-X) Copyright © 1999 Mosby, Inc. Terms and Conditions
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Fig. 4 Changes in the levels of surfactant protein A and mucin-like glycoprotein in induced sputum samples collected individually at the beginning (0 to 4 minutes) and end (16 to 20 minutes) of a 20-minute sputum induction. *Significantly different from the 0- to 4-minute interval (P < .05). Journal of Allergy and Clinical Immunology , DOI: ( /S (99)70374-X) Copyright © 1999 Mosby, Inc. Terms and Conditions
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Fig. 5 Cumulative data analysis from induced sputum samples collected individually at five 4-minute intervals during a 20-minute sputum induction in which volume-weighted averages were used to calculate the percentages of eosinophils and the concentrations of ECP in 12-minute versus 20-minute induced sputum samples. *Significantly different from the 12-minute sample (P < .05). Journal of Allergy and Clinical Immunology , DOI: ( /S (99)70374-X) Copyright © 1999 Mosby, Inc. Terms and Conditions
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