1. When you fall, pick something up."
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Title: Biology 3/9/07
Objectives:
To learn about the historical discoveries surrounding the genetic material, DNA.
Class Topics
Hand in W.S. 9.1 before the bell rings
Review Chapter 8 Test
Notes – Chapter 9
When you fall, pick something up."
Oswald Avery
Sunday, July 21, :56 PM

2. Class Assignments W.S. 9.1 3/9/07 Chapter 9 Quiz 1 3/13/07
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Class Assignments
What
By When
W.S /9/07
Chapter 9 Quiz 1 3/13/07
Due this class period
Due next class period
Due in the future

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Grade Sheet
2A – p. 157 (5 pts.)

4. Avery’s conclusion What’s the conclusion?
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Avery’s conclusion
Carbohydrase didn’t block transformation
Proteases didn’t block Transformation
Ribonuclease didn’t block transformation
Deoxyribonuclease blocked transformation
What’s the conclusion?
DNA is the genetic material of bacteria

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Avery’s experiment
Problem
Hypothesis
Procedure
Conclusion

6. From: http://ribonode.ucsc.edu/HuGen/01_80H/01_80Hlec6.html
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From:

7. Hershey-Chase Experiment
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Hershey-Chase Experiment
Alfred Hershey and Martha Chase worked with viruses to support the conclusion that DNA is the genetic material
The specific viruses were bacteriophages (phages)
infects bacteria
Contain both DNA and Protein

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Radioactive Isotopes
Isotope - same element (number of protons) but different numbers of neutrons
Unstable or radioactive (releases energy)
Used to follow the different elements throughout the experiment
Phosphorus-32 - DNA not protein
Sulfur-35 - protein not DNA

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Graphic taken from
the MIT Hypertext

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From:

11. Chapter 9 Quiz 1 Review Describe 1 of the 3 experiments
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Chapter 9 Quiz 1 Review
Describe 1 of the 3 experiments
Griffith, Avery, or Hershey/Chase
Problem
Hypothesis
Procedure
control & experimental groups, independent & dependent variables
Data
Conclusion

12. DNA Structure Monomer: Nucleotide Sugar (5C) - deoxyribose
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DNA Structure
Monomer: Nucleotide
Sugar (5C) - deoxyribose
Phosphate group
Nitrogenous base
Adenine - purine
Guanine - purine
Cytosine - pyrimidine
Thymine - pyrimidine

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14. Chargaff’s Rule Erwin Chargaff - 1950
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Chargaff’s Rule
Erwin Chargaff
Studied nucleotide composition of many DNA samples
Amount of each nucleotide differed in each sample
Amount of T = A and amount of G = C