1. Aggresome formation of STAT5A_ΔE18.
Aggresome formation of STAT5A_ΔE18. (A–F) HeLa cells stably expressing a STAT5A_ΔE18–EGFP fusion protein were treated with DMSO (A), 10 μM MG132 (B), 10 μM wortmannin (C) or 1 μM TSA (D) for 12 hours. Some cells were treated with 10 μM tubacin (E) or 10 μM niltubacin (F) for 8 hours before treatment with 5 μM MG132. The cells were then fixed, stained with DAPI to visualize the nuclei (blue) and observed by confocal laser microscopy. Scale bar: 10 μm. (G) Images of cells (n>100) from each treatment were examined and the STAT5A_ΔE18–EGFP signals were categorized into four types on the basis of size and distribution pattern: aggresomes, aggregates, diffuse distribution and no accumulation.
Yoshihisa Watanabe, and Masaki Tanaka J Cell Sci 2011;124:
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