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Fig. 3. Cell-autonomous loss of GABAergic interneurons in the OB of newborn and adult conditional GFRα1 mutants. Cell-autonomous loss of GABAergic interneurons in the OB of newborn and adult conditional GFRα1 mutants. (A) Representative images of the OB of newborn Gad67Cre;R1CGfx/fx conditional mutant and R1CGfx/fx control mice immunostained for markers of OB GABAergic interneurons: tyrosine hydroxylase (TH), Calbindin and Calretinin. Scale bar: 100 µm. (B) Representative images of the OB of P56 Gad67Cre;R1CGfx/fx conditional mutant and R1CGfx/fx control mice along the medial surface immunostained for markers of OB GABAergic interneurons: tyrosine hydroxylase (TH), Calbindin and Calretinin. Scale bar: 100 µm. (C) Quantification of the number of cells expressing TH, Calbindin (CB) and Calretinin (CR) in the OB of newborn Gad67Cre;R1CGfx/fx conditional mutants and R1CGfx/fx control mice. The values represent total number of cells in fields encompassing the entire OB. N=6 mice per group; *P<0.05; ***P< (D) Quantification of the number of cells expressing TH, Calbindin (CB) and Calretinin (CR) in the OB of P56 Gad67Cre;R1CGfx/fx conditional mutants and R1CGfx/fx control mice. The values represent number of cells counted in the glomerular layer of the medial surface of the OB (see the Materials and Methods for details). N=6 mice per group; **P<0.005; ***P< ONL, olfactory nerve layer; GL, glomerular layer; EPL, external plexiform layer; MI, mitral cell layer; GR, granule cell layer. Sabrina Zechel et al. Biology Open 2018;7:bio033753 © Published by The Company of Biologists Ltd
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