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Published byYulia Iskandar Modified over 5 years ago
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Bacteria trigger oxygen radical release and sperm lipid peroxidation in in vitro model of semen inflammation Monika Fraczek, Ph.D., Anna Szumala-Kakol, Ph.D., Piotr Jedrzejczak, M.D., Ph.D., Marzena Kamieniczna, Ph.D., Maciej Kurpisz, M.D., Ph.D. Fertility and Sterility Volume 88, Issue 4, Pages (October 2007) DOI: /j.fertnstert Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions
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Figure 1 Modulatory effect of spermatozoa (1 × 106/mL) from (A) swim-up, (B) 90% Percoll, and (C) 47% Percoll fractions on reactive oxygen intermediate generation exerted by white blood cells (WBC) (1 × 106/mL) incubated without (black) or with the following bacteria: E. coli (blue), S. haemolyticus (green), Str. oralis (red), B. ureolyticus (violet), and U. urealyticum (yellow). The results are expressed as median ± average deviation. ∗P<.05; ∗∗P<.01; calculated using Friedman test, compared with respective baseline. Fraczek. Bacteriospermia and oxidative stress. Fertil Steril 2007. Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions
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Figure 2 Malondialdehyde (MDA) concentration in spermatozoal fractions. The results are expressed as median ± average deviation. ∗P<.05; ∗∗P<.01; calculated using Kruskal-Wallis test, compared with sperm isolated by 90% Percoll fraction. Fraczek. Bacteriospermia and oxidative stress. Fertil Steril 2007. Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions
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Figure 3 Influence of selected bacterial strains on malondialdehyde (MDA) concentration in different spermatozoal fractions (A) without white blood cells (WBC) and (B) after incubation with WBC. The results are expressed as median ± average deviation. ∗P<.05; ∗∗P<.01; calculated using Friedman test, compared with respective controls. Fraczek. Bacteriospermia and oxidative stress. Fertil Steril 2007. Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions
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