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Peptide mass spectra of SUMO target proteins.
Peptide mass spectra of SUMO target proteins. Peptide mass spectra of proteins were quantified, and peptides matching the control fraction (Arg0), the SUMO-1 enriched fraction (Arg6), and the SUMO-2 enriched fraction (Arg10) are indicated. Chromodomain helicase DNA-binding protein 1 is an example of a non-sumoylated protein (A), chromodomain helicase DNA-binding protein 2 (B) and RanGAP1 (C) are examples of preferential SUMO-1 targets, PML (E) and Ataxin-2-related domain protein (RDP) (F) are examples of preferential SUMO-2 targets, and SART1 is an example of a protein that is conjugated to both SUMO family members (D). Ctrl, control. Alfred C. O. Vertegaal et al. Mol Cell Proteomics 2006;5: © 2006 The American Society for Biochemistry and Molecular Biology
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