1. Stress and serotonin system
David Chen
Stress and serotonin system

2. What is serotonin? Neurotransmitter(神经递质)
Also called 5-hydrotrptamine(5-HT)5-羟色胺
Synthesized from tryptphan

3. How does this system work?
Fig. from Genetic variation in cortico-amygdala serotonin function and risk for stress-related disease

4. Distribution of serotonergic neurons in our brain
mPFC内侧前额叶
Amygdala杏仁体
DRN中缝背核
Fig. from Genetic variation in cortico-amygdala serotonin function and risk for stress-related disease

5. Relation between serotonin and stress
Exposure to stressors … serotonin neuron activity is increased…
--Excerpted from Medial prefrontal cortex determines how stressor controllability affects behavior and dorsal raphe nucleus
…at all levels of the serotonin system…can influence … the response to stress.
--Excerpted from Genetic variation in cortico-amygdala serotonin function and risk for stress-related disease

6. Release
Synthesis
Vesicular packaging
Degradation
Receptor

7. Compromised by chronic stress leading to ‘learned helplessness’（习得性无助）
5-HT1A-R
Autoreceptor (自受体)on the soma and dendrites of serotonin cells
--Excerpted from Immunohistochemical evidence of the existence of 5-HT1A autoreceptors on serotoninergic neurons in the midbrain raphe nuclei
Whose activation inhibits DRN neuronal firing and consequent forebrain release
--Excerpted from Direct Immunohistochemical evidence of
the existence of 5-HT1A autoreceptors on serotoninergic neurons in the midbrain raphe nuclei.
Compromised by chronic stress leading to ‘learned helplessness’（习得性无助）
--excerpted from Learned helplessness

8. Method Forced swim test Tail suspension test Gene knockout
Evaluation of antidepressants（抗抑郁药）… aimed at rendering or preventing depressive-like states
--Excerpted from The mouse forced swim test.
Tail suspension test
Gene knockout

9. Reference
[1] Sotelo C, Cholley B, El Mestikawy S, Gozlan H, Hamon M. Direct Immunohistochemical evidence of the existence of 5-HT1A autoreceptors on serotoninergic neurons in the midbrain raphe nuclei. EurJ Neurosci 1990;2:1144–1154.
[2] Seligman ME. Learned helplessness. Annu Rev Med 1972;23:407–412
[3] C Adem et al. The Mouse Forced Swim Test J Vis Exp. 2012 Jan 29;(59):e3638
[4] Andrew Holmes Genetic variation in cortico-amygdala serotonin function and risk for stress-related disease Neurosci Biobehav Rev September ; 32(7): 1293–1314. doi: /j.neubiorev
[5] Clifford EM, Gartside SE, Umbers V, Cowen PJ, Hajos M, Sharp T. Electrophysiological and neurochemical evidence that pindolol has agonist properties at the 5-HT1A autoreceptor in vivo. Br J Pharmacol 1998;124:206–212.
[6] Amat J, Baratta MV, Paul E, Bland ST, Watkins LR, Maier SF. Medial prefrontal cortex determines how stressor controllability affects behavior and dorsal raphe nucleus. Nat Neurosci 2005;8:365–371

10. Any suggestions will be highly appreciated!