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Effects of mifepristone on expression of endothelial nitric oxide synthase in human endometrium during the implantation phase Xiaoxi Sun, M.D., Xiaoyan Qiu, M.D., Ph.D., Kristina Gemzell-Danielsson, M.D., Ph.D. Fertility and Sterility Volume 80, Issue 6, Pages (December 2003) DOI: /j.fertnstert
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FIGURE 1 Immunhistochemical location of endothelial nitric oxide synthase (eNOS) in human endometrium during the implantation phase. (A) Endothelial NOS immunoreactivity in control cycles. Staining occurred in vascular endothelium and glandular epithelium cells but not stromal cells. (B) Endometrium after mifepristone treatment. The eNOS immunostaining is markedly diminished in glandular cells but not vascular endothelium. (C) Negative control for eNOS. Scale bar =100 μm. Sun. eNOS expression in the human endometrium. Fertil Steril 2003. Fertility and Sterility , DOI: ( /j.fertnstert )
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FIGURE 2 (A) Western blot analysis of human endometrium during the implantation phase from control (n =4) (lanes 1 to 4) and mifepristone-treated specimens (n =4) (lanes 5 to 8). The expected endothelial nitric oxide synthase band is seen at 140 kD. Lane 9 is a positive control from human umbilical vein epithelium cell line. (B) Expression of endothelial nitric oxide synthase protein in control (open bar) and mifepristone-treated (closed bar) specimens. Each band was densitometrically scanned and corrected against human umbilical vein epithelium cell line and is represented as the intensity ratio of Western blot. Each column represents the mean of the intensity ratio. Bars show the SD. Sun. eNOS expression in the human endometrium. Fertil Steril 2003. Fertility and Sterility , DOI: ( /j.fertnstert )
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FIGURE 3 (A) Reverse transcription-polymerase chain reaction for eNOS using total RNA extracted from endometrial control (lanes 1 to 4) and mifepristone-treated specimens (lanes 5 to 8). Lane M is a 100–base pair ladder, lane 9 is a negative control from which complementary DNA was omitted, and lane 10 is a positive control from human umbilical vein epithelium cell line. (B) Expression of endothelial nitric oxide synthase messenger RNA during the implantation phase in human endometrium control (open bar) and mifepristone-treated (closed bar) specimens. Each band was densitometrically scanned and corrected against 28S and is represented as the intensity ratio of RT-PCR. Each column represents the mean of the intensity ratio. Bars show the SD. Sun. eNOS expression in the human endometrium. Fertil Steril 2003. Fertility and Sterility , DOI: ( /j.fertnstert )
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