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Volume 2, Issue 4, Pages (July 2009)

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1 Volume 2, Issue 4, Pages 711-723 (July 2009)
Expression, Imprinting, and Evolution of Rice Homologs of the Polycomb Group Genes  Luo Ming , Platten Damien , Chaudhury Abed , Peacock W.J. , Dennis Elizabeth S.   Molecular Plant  Volume 2, Issue 4, Pages (July 2009) DOI: /mp/ssp036 Copyright © 2009 The Authors. All rights reserved. Terms and Conditions

2 Figure 1 Rice PcG Gene Expression Shown by RT–PCR.
Sh, sheath; P, panicle; O, ovary; A, anther; 2d, 2-day-old seeds; 4d, 4-day-old endosperm; R, roots; Yl, young leaf. OsFIE1 is only expressed in endosperm. Other genes are expressed widely. Molecular Plant 2009 2, DOI: ( /mp/ssp036) Copyright © 2009 The Authors. All rights reserved. Terms and Conditions

3 Figure 2 Parental Expression of the Rice PcG Genes in Endosperm.
The arrows indicate the polymorphism between parents. NIP, Nipponbare. NIP was pollinated with IR64. RT–PCR was conducted on the 5-day-old endosperm isolated from hybrid and parents. Sequencing was carried out on the RT-products and their subclones. The sequencing trace files are from the direct sequencing of RT–PCR products. The maternal and paternal ratios are the counts of each individual sub-clone of RT–PCR products from hybrid endosperm. Molecular Plant 2009 2, DOI: ( /mp/ssp036) Copyright © 2009 The Authors. All rights reserved. Terms and Conditions

4 Figure 3 Evolutionary Relationships of E(z) Proteins from 50 Taxa.
The rice E(z) homologs are highlighted. Molecular Plant 2009 2, DOI: ( /mp/ssp036) Copyright © 2009 The Authors. All rights reserved. Terms and Conditions

5 Figure 4 Evolutionary Relationships of Su(z)12 Proteins from 37 Taxa.
The rice Su(z)12 homologs are highlighted. Molecular Plant 2009 2, DOI: ( /mp/ssp036) Copyright © 2009 The Authors. All rights reserved. Terms and Conditions

6 Figure 5 Evolutionary Relationships of ESC Proteins from 54 Taxa.
The rice FIE proteins are highlighted. Molecular Plant 2009 2, DOI: ( /mp/ssp036) Copyright © 2009 The Authors. All rights reserved. Terms and Conditions

7 Figure 6 Putative Phenotypes and Pseudoseed Formation of a T-DNA Insertion Mutant of OsEMF2b. (A) A homozygous mutant and a wild-type grown in long days; the shorter plant was a homozygous mutant at flowering and the taller wild-type was at the vegetative stage. (B) Abortive florets in homozygous mutants. (C) A panicle of a homozygous mutant (left) has fewer florets than the panicle of a wild-type (right). (D) Multiple-fused ovaries were seen occasionally. (E) A floret appeared to be normal but without pollen in a homozygous mutant. (F) A wild-type floret with pollen being released. (G) A 5-day-old fertilized wild-type seed. The arrow indicates the boundary between endosperm and proliferated nucellus cells. (H) A pseudoseed showing proliferation of nucellus cells in a OsEMF2b heterozygote after emasculation. (I) A 9-day-old wild-type seed with starchy endosperm. (J) A late pseudoseed showing nucellus proliferation in a wild-type plant after emasculation. (K, L) Pseudoseeds from emasculated panicles; one is from a wild-type (K), another is from a heterozygous Osemf2b mutant (L). Bars in (B–F)  =  2.5 mm; bars in (G–J)  =  0.25 mm; bars in (K, L)  =  6 mm. Molecular Plant 2009 2, DOI: ( /mp/ssp036) Copyright © 2009 The Authors. All rights reserved. Terms and Conditions


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