1. IRF4 expression is modulated by the signaling of IL-2 family cytokines.
IRF4 expression is modulated by the signaling of IL-2 family cytokines. (A) Purified WT C57BL/6 CD8+ T cells were activated in vitro in the presence or absence of human IL-2 (150 U/ml). IRF4 expression in CD8+ T cells was determined using ICS on different days. (B) Purified WT C57BL/6 CD8+ T cells were activated in vitro in the presence or absence of IL-15 or IL-7 (50 ng/ml). IRF4 expression in CD8+ T cells was determined using ICS at day 2 postactivation. (C) Purified OT-I T cells were activated in vitro with N4, T4, or Q4H7 peptides in the presence or absence of human IL-2 (150 U/ml). IRF4 expression in CD8+ T cells was determined using ICS on different days. (D) Purified WT C57BL/6 CD8+ T cells were activated for 1 d and then cells were transduced with control retrovirus or retrovirus harboring STAT5-CA. IRF4 expression in transduced CD8+ T cells was determined using ICS at days 1 and 2 posttransduction. Data are representative of at least three experiments (A–C) or two experiments (D).
Su Huang et al. ImmunoHorizons 2017;1:92-100
Copyright © 2017 The Authors