1. Diabetes-induced impairment in angiogenic capacity in subcutaneous sponge implants.
Diabetes-induced impairment in angiogenic capacity in subcutaneous sponge implants. Sterile sponge discs were implanted subcutaneously into control and GK diabetic rats that had been anesthetized with ketamine and xylazine. Sponges were retrieved 10–12 days post-implantation, bisected and either embedded and sectioned or frozen in liquid nitrogen and used for microscopic or biochemical analysis of angiogenic capacity. (A) Representative photomicrographs of hematoxylin-eosin-stained sponge sections revealing fibrovascular invasion. Arrows denote the presence of large and small blood vessels. (B) Representative photomicrographs of CD31 immunofluorescence staining. (C) Quantitation of CD31 fluorescence intensity from images shown in B. (D) Hemoglobin content in the sponges, as determined by using the Drabkin reagent. (E) A proliferation response in sponge implants was assessed by immunostaining for BrdU. (F) Picrosirius-Red-stained sponge sections were viewed under a polarized-light microscope and revealed the presence of red tightly packed mature collagen fibers in sponges from control mice, but these were present to a much lesser extent in sponges from diabetic mice. (G) Collagen type 1 mRNA content from sponges, as assessed by qRT-PCR analyses. ‘C’, control; ‘D’, diabetic. Results are expressed as means±s.e.m. from three independent experiments. *Significantly different from corresponding control values at P≤0.05.
Milad S. Bitar, and Fahd Al-Mulla Dis. Model. Mech. 2015;8:65-80
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