1. Lecture 11 Slides rh

3. electron transport chain
fig19-16

4. fig19-17

5. proton-motive force
23.6 kJ/mole H+

6. the coupling question…
chemiosmotic hypothesis!

7. the coupling question…
chemiosmotic hypothesis!

9. fig19-19

10. oxidative phosphorylation experiment
measure
this
add
this

11. fig19-20

12. oxidative phosphorylation experiment
ADP
+ Pi
ATP
measure
this
add
this

13. fig19-20

14. oxidation and phosphorylation are coupled!!!
fig19-20

15. oxidation and phosphorylation are coupled!!!
fig19-20

16. oxidative phosphorylation experiment
ADP
+ Pi
ATP
measure
this
add
this

17. oxidative phosphorylation experiment
ADP
+ Pi
ATP
remove
this
this
stops

18. oxidative phosphorylation experiment
ADP
+ Pi
ATP
block
ATPase
this
stops

19. weak acid uncouplers
high H+
low H+

20. Weak acid
uncouplers
fig19-21

21. Chemical uncouplers
fig19-21

22. Chemical uncouplers
fig19-21

23. Chemical uncoupler experiment
fig19-20

24. From ETC and OxPhos

25. Proton
gradient
experiment
fig19-22

26. ATP synthase: the F1 subunit

27. ATP and ADP are
in rapid equilibrium
in the F1 ATPase
fig19-23

28. Energetics of ATP and ADP in F1 ATPase…
fig19-24

29. The “binding-change” model for ATP synthase
fig19-25

30. The “binding-change” model for ATP synthase
fig19-25

31. The “binding-change” model for ATP synthase
fig19-26

32. The F1 complex of the ATPase…
fig19-25

34. The F0F1 complex
fig19-25

35. The F0F1 complex
membrane
soluble
fig19-25

36. The F0F1 complex of the ATPase…
fig19-25

37. The F0F1 complex of the ATPase…
fig19-25

39. A direct test of
the rotary
mechanism
fig19-25
Noji et al. 1997

40. A direct test of
the rotary
mechanism

41. Snapshots from the direct test of rotary mechanism…
fig19-27

42. The movie version of the book

43. Bacterial generation of H+ gradient by respiratory chain
an ancient mode of ATP production

44. “There’s more than one way to shrink a gradient…”
fig19-39

45. Inner membrane translocases (or transporters)
fig19-28

47. Glycerol 3-P shuttle
fig19-30

48. Malate-aspartate shuttle
eeeeek!
fig19-29

49. Malate-aspartate shuttle
NADH
NAD+

50. Malate-aspartate shuttleX

51. Malate-aspartate shuttle viewed as e- carrying process
NAD+
NAD+
NADH
NADH
OAA
OAA

52. Malate-aspartate shuttle viewed as e- carrying process
with set of transaminations between OAA/Asp, and aKG/Glu
malate
NADH
NAD+
OAA
Asp
aKG
Glu

53. Malate-aspartate shuttle viewed as e- carrying process
NAD+
NAD+
NADH
NADH
OAA
OAA

54. with set of transaminations between OAA/Asp, and aKG/Glu

55. with set of transaminations between OAA/Asp, and aKG/Glu

56. with set of transaminations between OAA/Asp, and aKG/Glu
NH3 group
from Asp to
aKG
NH3 group
from Glu to
OAA

57. Malate-aspartate shuttle viewed as e- carrying process
with set of transaminations between OAA/Asp, and aKG/Glu
malate
NADH
NAD+
OAA
Asp
aKG
Glu

58. Malate-aspartate shuttle
fig19-29

59. Nature’s
electric
blanket
thermogenin
fig19-34

60. regulation of
ATP producing
pathways
fig19-33

61. regulation of
ATP producing
pathways
fig19-33

62. regulation of
ATP producing
pathways
fig19-33