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Fig. 4. One-dimensional gel electrophoresis of DIMs

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1 Fig. 4. One-dimensional gel electrophoresis of DIMs
Fig. 4. One-dimensional gel electrophoresis of DIMs.(a) Electrophoretic profile of fractions of the Optiprep density gradients. One-dimensional gel electrophoresis of DIMs.(a) Electrophoretic profile of fractions of the Optiprep density gradients. Fractions were run in two 10% polyacrylamide gels (10 slots for each gel). The floating material was recovered in fractions 6–7 in all experiments, irrespective of detergent/protein ratio. The same volume was loaded in each lane. (b) Graph showing the percentage of protein recovered in DIMs with respect to P2, at increasing detergent/protein ratios (3<n<11). Error bars indicate standard errors. (c) Electrophoretic profile of DIMs obtained with detergent/protein ratios of 2:1 to 5:1 compared with that of P2. 5 µg protein was loaded in each lane. Polypeptides indicated by the asterisks disappeared with increasing detergent/protein ratio. (d) Electrophoretic profile of DIMs obtained with a detergent/protein ratio of 8:1, compared with P2. Polypeptides indicated by the asterisks, arrows and brackets increased with respect to P2. 5 µg of protein was loaded in each lane. Alessandra Moscatelli et al. Biology Open 2015;bio © Published by The Company of Biologists Ltd

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