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Dosage compensation in birds
Heather A McQueen, Derek McBride, Gino Miele, Adrian P Bird, Michael Clinton Current Biology Volume 11, Issue 4, Pages (February 2001) DOI: /S (01)
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Figure 1 FISH mapping of Z chromosome genes. (a–c) Examples of hybridizations to metaphase chromosomes, with gene names written in the same colors as their hybridized probe signals. In each case a colored plot shows the chromosomal position of the red and green probe signals as a percentage of the entire chromosome, represented in blue. (a) follistatin maps to Zp2.2–2.3, and the signal is shown at approximately 20% from p to q. (b) ScII maps to Zq1.5–1.6, and the signal is shown at approximately 80% from p to q. (c) ScII mapping relative to aldolase B (Zq1.5, 75% pq) and CHRNB3 (Zq1.3, 62% pq). The Z chromosome plot shown is drawn from q to p. (d)ScII location between aldolase B and the previously mapped IREBP gene is shown by hybridization to an interphase nucleus. (e) Cartoon of the Z chromosome shows physical locations for the nine mapped genes used in the expression analysis. Percentage distances along the chromosome from p to q were derived from multiple experiments Current Biology , DOI: ( /S (01) )
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Figure 2 Expression analysis for nine Z chromosome genes and one autosomal gene on individual male and female embryos. (a) Relative expression levels for eight Z chromosome genes and growth hormone gene, corrected for GAPDH expression, in the same six female and six male day 4 embryos by SYBR Green real-time quantitative PCR analysis. Females (left) are shown in pink, and males (right) are shown in blue. Expression levels in each case are relative to the first female, whose value was scaled to 1.0. Male:female (m:f) values are given. The first male value for the ScII plot was 6.0 and could not therefore be plotted within the chart. Standard deviations of the mean values are shown. (b) Relative expression levels for 22 male and 16 female day 3 and 4 samples for follistatin and ScII genes. The expression levels are corrected for GAPDH expression by TaqMan analysis. Male:female (m:f) values for combined results from days 3 and 4 are given. One male day 4 ScII value was 3.9 and could not be plotted on the chart Current Biology , DOI: ( /S (01) )
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