1. Reaction of a peptide-αthioester with an N-terminal Asp(β-HA)-peptide yields a peptide-αO-acyl hydroxamic acid isopeptide under mild aqueous conditions.
Reaction of a peptide-αthioester with an N-terminal Asp(β-HA)-peptide yields a peptide-αO-acyl hydroxamic acid isopeptide under mild aqueous conditions. Reaction scheme and LC-MS data showing conversion of thioester 2 to O-acyl hydroxamic acid isopeptide 1, by treatment with N-terminal Asp(β-HA)-peptide 3, at pH 9.
Daniel L. Dunkelmann et al. PNAS 2018;115:15:
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