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Published byΔάμαλις Κανακάρης-Ρούφος Modified over 5 years ago
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Fig. 7. Representative images of control (Cas9+GFP) and Cas9+gRNA+GFP co-injected embryos on day 4 of culture, showing nuclear-imported GFP (green) and DNA counter-staining with DAPI (blue). Representative images of control (Cas9+GFP) and Cas9+gRNA+GFP co-injected embryos on day 4 of culture, showing nuclear-imported GFP (green) and DNA counter-staining with DAPI (blue). (A) 8-cell stage control embryo. (B,C) Cas9+gRNAs+GFP injected embryos showing abnormal nuclear morphology (arrows) at 6- and 8-cell stage, respectively. Jiude Mao et al. Biology Open 2018;7:bio035717 © Published by The Company of Biologists Ltd
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