1. In 6CFSMEo- cells, the NMD factor UPF1 colocalizes with NMD substrates under cytoskeleton disruptor treatment. 6CFSMEo- cells were transfected with constructs expressing GPx1 Ter and YFP–UPF1 and then incubated with DMSO, cytochalasin D (CytoD), JPK, colchicine (COL) or Taxotere (TAX) for 48 h.
In 6CFSMEo- cells, the NMD factor UPF1 colocalizes with NMD substrates under cytoskeleton disruptor treatment. 6CFSMEo- cells were transfected with constructs expressing GPx1 Ter and YFP–UPF1 and then incubated with DMSO, cytochalasin D (CytoD), JPK, colchicine (COL) or Taxotere (TAX) for 48 h. A fluorescence in situ hybridization (FISH) assay was performed to detect the cellular localization of GPx1 Ter mRNA. Nuclei are stained in blue with Hoechst stain. Green arrowheads indicate UPF1 cytoplasmic foci; red arrowheads indicate mRNA cytoplasmic GPx1 mRNA foci; orange arrowheads indicate colocalization of the UPF1 factor and NMD substrates. The histogram represents the percentage of colocalization between UPF1 foci and GPx1 Ter. Cells (mean±s.d.; n=10) from three different experiments were counted for each condition.
Jieshuang Jia et al. J Cell Sci 2017;130:
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