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Detection of nuclear foci by FISH on frozen sections of diaphragm muscle prepared from 2-month-old DMSXL mice. Detection of nuclear foci by FISH on frozen.

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Presentation on theme: "Detection of nuclear foci by FISH on frozen sections of diaphragm muscle prepared from 2-month-old DMSXL mice. Detection of nuclear foci by FISH on frozen."— Presentation transcript:

1 Detection of nuclear foci by FISH on frozen sections of diaphragm muscle prepared from 2-month-old DMSXL mice. Detection of nuclear foci by FISH on frozen sections of diaphragm muscle prepared from 2-month-old DMSXL mice. Using the Cy3-labeled CAG-repeats PNA-probe, the RNA inclusions are labeled in red within the nuclei of muscle fibers stained in blue with DAPI. (A) Low magnification image shows numerous nuclei stained in blue surrounding the diaphragm muscle fibers. (B,C) At higher magnification the presence of one or more foci inside the nuclei is easily observed. Scale bars: 20 μm (A); 5 μm (B,C). Petrica-Adrian Panaite et al. Dis. Model. Mech. 2013;6: © Published by The Company of Biologists Ltd

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