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Fig. 3 Cytosolic delivery of fluorescent proteins.

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Presentation on theme: "Fig. 3 Cytosolic delivery of fluorescent proteins."— Presentation transcript:

1 Fig. 3 Cytosolic delivery of fluorescent proteins.
Cytosolic delivery of fluorescent proteins. (A) P4 in the cytosolic delivery of R-PE into HeLa cells for 4 hours. Commercial reagents PULSin and TransEx were used as positive controls. (B) Quantitative analysis of the protein internalization in (A) by flow cytometry. The dose of R-PE in each well was 0.5 μg. P4 in the cytosolic delivery of (C) negatively charged GFP, YFP, and RFP into HeLa cells for 4 hours. The dose of proteins in each well was 3 μg. P4 in the cytosolic delivery of (D) positively charged Cyt C, lysosome, and trypsin into HeLa cells for 4 hours. The doses of proteins in each well were 6, 6, and 3 μg, respectively, and the dose of P4 for all the experiments was 8 μg. Chongyi Liu et al. Sci Adv 2019;5:eaaw8922 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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