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Potential role of interleukin-1 in allergen-induced late asthmatic reactions in guinea pigs: Suppressive effect of interleukin-1 receptor antagonist on.

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Presentation on theme: "Potential role of interleukin-1 in allergen-induced late asthmatic reactions in guinea pigs: Suppressive effect of interleukin-1 receptor antagonist on."— Presentation transcript:

1 Potential role of interleukin-1 in allergen-induced late asthmatic reactions in guinea pigs: Suppressive effect of interleukin-1 receptor antagonist on late asthmatic reaction  Shinji Okada, MD, Hiroshi Inoue, MD, Kohei Yamauchi, MD, Hideya Iijima, MD, Yuichi Ohkawara, MD, Tamotsu Takishima, MD, Kunio Shirato, MD  Journal of Allergy and Clinical Immunology  Volume 95, Issue 6, Pages (June 1995) DOI: /S (95) Copyright © 1995 Mosby, Inc. Terms and Conditions

2 FIG. 1 Immunostaining of the airways taken from guinea pigs sensitized and exposed to Ascaris antigen. A, The airway 2 hours after the antigen exposure was immunostained with anti-IL-1-β antibody. Cells in the bronchial epithelium and a few cells in the submucosal layer were stained in red with streptoavidin-biotin/alkaline phosphatase and Fast Red substrates (K699, Dako). B, The same airway section as shown in A was immunostained with anti-IL-1β-antibody–absorbed solution. No cells were stained in red. C, The airway 8 hours after antigen exposure. Cells in the epithelium, and a few cells in the peribronchiolar layer (arrow) demonstrated positive staining for IL-1β. D, The airway 24 hours after antigen exposure. No cells showed positive staining for IL-1β, although cell infiltrations to the submucosal layer were observed. Counterstaining was performed with hematoxilin. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) ) Copyright © 1995 Mosby, Inc. Terms and Conditions

3 FIG. 1 Immunostaining of the airways taken from guinea pigs sensitized and exposed to Ascaris antigen. A, The airway 2 hours after the antigen exposure was immunostained with anti-IL-1-β antibody. Cells in the bronchial epithelium and a few cells in the submucosal layer were stained in red with streptoavidin-biotin/alkaline phosphatase and Fast Red substrates (K699, Dako). B, The same airway section as shown in A was immunostained with anti-IL-1β-antibody–absorbed solution. No cells were stained in red. C, The airway 8 hours after antigen exposure. Cells in the epithelium, and a few cells in the peribronchiolar layer (arrow) demonstrated positive staining for IL-1β. D, The airway 24 hours after antigen exposure. No cells showed positive staining for IL-1β, although cell infiltrations to the submucosal layer were observed. Counterstaining was performed with hematoxilin. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) ) Copyright © 1995 Mosby, Inc. Terms and Conditions

4 FIG. 1 Immunostaining of the airways taken from guinea pigs sensitized and exposed to Ascaris antigen. A, The airway 2 hours after the antigen exposure was immunostained with anti-IL-1-β antibody. Cells in the bronchial epithelium and a few cells in the submucosal layer were stained in red with streptoavidin-biotin/alkaline phosphatase and Fast Red substrates (K699, Dako). B, The same airway section as shown in A was immunostained with anti-IL-1β-antibody–absorbed solution. No cells were stained in red. C, The airway 8 hours after antigen exposure. Cells in the epithelium, and a few cells in the peribronchiolar layer (arrow) demonstrated positive staining for IL-1β. D, The airway 24 hours after antigen exposure. No cells showed positive staining for IL-1β, although cell infiltrations to the submucosal layer were observed. Counterstaining was performed with hematoxilin. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) ) Copyright © 1995 Mosby, Inc. Terms and Conditions

5 FIG. 1 Immunostaining of the airways taken from guinea pigs sensitized and exposed to Ascaris antigen. A, The airway 2 hours after the antigen exposure was immunostained with anti-IL-1-β antibody. Cells in the bronchial epithelium and a few cells in the submucosal layer were stained in red with streptoavidin-biotin/alkaline phosphatase and Fast Red substrates (K699, Dako). B, The same airway section as shown in A was immunostained with anti-IL-1β-antibody–absorbed solution. No cells were stained in red. C, The airway 8 hours after antigen exposure. Cells in the epithelium, and a few cells in the peribronchiolar layer (arrow) demonstrated positive staining for IL-1β. D, The airway 24 hours after antigen exposure. No cells showed positive staining for IL-1β, although cell infiltrations to the submucosal layer were observed. Counterstaining was performed with hematoxilin. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) ) Copyright © 1995 Mosby, Inc. Terms and Conditions

6 FIG. 2 Cells in BALF. A, Cells from nonsensitized and nonchallenged guinea pigs demonstrated very weak staining for IL-1β. B, Cells from normal guinea pigs (same as in A) were incubated with lipopolysaccharide (10 μg/ml, 2 hours). Macrophages showed stronger staining than in A. C, One hour after challenge, macrophages showed weak staining, but the intensity was stronger than in A. D, Six hours after antigen challenge, macrophages showed staining as strong as that in B. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) ) Copyright © 1995 Mosby, Inc. Terms and Conditions

7 FIG. 2 Cells in BALF. A, Cells from nonsensitized and nonchallenged guinea pigs demonstrated very weak staining for IL-1β. B, Cells from normal guinea pigs (same as in A) were incubated with lipopolysaccharide (10 μg/ml, 2 hours). Macrophages showed stronger staining than in A. C, One hour after challenge, macrophages showed weak staining, but the intensity was stronger than in A. D, Six hours after antigen challenge, macrophages showed staining as strong as that in B. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) ) Copyright © 1995 Mosby, Inc. Terms and Conditions

8 FIG. 2 Cells in BALF. A, Cells from nonsensitized and nonchallenged guinea pigs demonstrated very weak staining for IL-1β. B, Cells from normal guinea pigs (same as in A) were incubated with lipopolysaccharide (10 μg/ml, 2 hours). Macrophages showed stronger staining than in A. C, One hour after challenge, macrophages showed weak staining, but the intensity was stronger than in A. D, Six hours after antigen challenge, macrophages showed staining as strong as that in B. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) ) Copyright © 1995 Mosby, Inc. Terms and Conditions

9 FIG. 3 Time course of changes in R1 after the challenge in the three groups; antigen challenged with IRAP pretreatment (group I, ●, n = 6), without pretreatment (group II, ○, n = 6), and saline-challenged (IRAP pretreatment [−s], group III, ▵, n = 6) groups. Significant increases in R1 in both the immediate and late phases were observed in antigen-challenged guinea pigs ○, compared with saline-challenged guinea pigs ▵ (p < 0.05). Increase in R1 was significantly reduced in the late phase (2 to 6 hours) after antigen challenge in group I (●) compared with group II (○) (detected with Mann-Whitney U test for change in R1 versus time area under the curve). Further, significance was observed at the 5-hour time point. *p < R L, Pulmonary resistance. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) ) Copyright © 1995 Mosby, Inc. Terms and Conditions

10 FIG. 4 Cellular components in BALF 6 hours after challenge. Significant increases in eosinophil and lymphocyte cell counts were observed after antigen challenge (p < 0.05). However, no differences in cell counts were observed between IRAP-pretreated and nontreated groups. No difference in macrophage or neutrophil cell count was observed among three groups. BAL, Bronchoalveolar lavage; Mø, macrophages. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) ) Copyright © 1995 Mosby, Inc. Terms and Conditions

11 FIG. 5 Eosinophil density distribution in the IRAP-pretreated guinea pigs (●, n = 5) and in the IRAP pretreatment (−) guinea pigs (○, n = 5). IRAP pretreatment shifted eosinophil density from hypodense to hyperdense. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) ) Copyright © 1995 Mosby, Inc. Terms and Conditions

12 FIG. 6 Time course of percent changes in R1 after instillation of rhIL-1β (1 μg/kg, ●, n = 4) or the same concentration of bovine serum albumin (○, n = 4). No significant change in R1 was observed after the instillation of rhIL-1β. No significant difference in percent change in R1 was observed between the two groups. BSA, Bovine serum albumin; R L, Pulmonary resistance. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) ) Copyright © 1995 Mosby, Inc. Terms and Conditions

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