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Published byΆκανθα Μάγκας Modified over 5 years ago
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miR-200c/141 overexpression in LS-8 cells induces E-cad expression and cell-cell adhesion.
miR-200c/141 overexpression in LS-8 cells induces E-cad expression and cell-cell adhesion. (A-C) Morphology of LS-8 cells that were left untreated (A), transduced with empty vector (B), or transduced with lentiviral miR-200c/141 (C). (D-F) Immunofluorescence staining shows increased levels of E-cad and cell-cell adherence with miR-200c/141 expression (F). (G-I) DAPI staining of LS-8 nuclei. (J-L) Overlay of E-cad and DAPI stains. (M) Western blot of E-cad expression in LS-8 cells transduced with empty vector or lentiviral miR-200c/141, shown at two concentrations of cell lysate. Gapdh provided a loading control. Huojun Cao et al. Development 2013;140: © Published by The Company of Biologists Ltd
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