1. Modulation of AMPA receptor GluR1 subunit phosphorylation in neurons by the intravenous anaesthetic propofol 
M. Haines, L.M. Mao, L. Yang, A. Arora, E.E. Fibuch, J.Q. Wang 
British Journal of Anaesthesia 
Volume 100, Issue 5, Pages (May 2008)
DOI: /bja/aen051
Copyright © 2008 British Journal of Anaesthesia Terms and Conditions

2. Fig 1
Representative immunoblots depicting the selectivity of phospho- and site-specific antibodies. Addition of an anti-pGluR1-Ser831 or anti-pGluR1-Ser845 antibody produced a single band at a molecular weight predicted for the size of GluR1 (106 kDa) in immunoblots with striatal samples (L1 and L2). No immunoblot band was present in samples pretreated with a lambda protein phosphatase (400 units ml−1, 4 h) which induced dephosphorylation of phosphorylated GluR1 subunits (L3 and L4).
British Journal of Anaesthesia  , DOI: ( /bja/aen051)
Copyright © 2008 British Journal of Anaesthesia Terms and Conditions

3. Fig 2
Effects of propofol incubation on serine phosphorylation of GluR1 subunits in cultured rat striatal neurons. Propofol at five different concentrations (0.1, 1, 3, 10, and 30 μM) was added to cultures for 5 min. Representative immunoblots are shown above the quantified data of four proteins analysed from separate experiments [mean (sem), n = 8–10]. Note that propofol incubation enhanced cellular pGluR1-Ser845, but not pGluR1-Ser831 and GluR1 levels, in a concentration-dependent fashion. Fold basal was determined by dividing band intensities obtained at different propofol concentrations by basal band intensity obtained at 0 μM of propofol. *P < 0.05 vs basal levels.
British Journal of Anaesthesia  , DOI: ( /bja/aen051)
Copyright © 2008 British Journal of Anaesthesia Terms and Conditions

4. Fig 3
Effects of propofol incubation at different concentrations on serine phosphorylation of GluR1 subunits in cultured rat cortical neurons. Propofol at five different concentrations (0.1, 1, 3, 10, and 30 μM) was added to cultures for 5 min. The quantified data of four proteins were analysed from separate experiments [mean (sem), n = 5–9]. Note that propofol incubation enhanced cellular pGluR1-Ser845, but not pGluR1-Ser831 and GluR1 levels, in a concentration-dependent fashion. *P < 0.05 vs basal levels.
British Journal of Anaesthesia  , DOI: ( /bja/aen051)
Copyright © 2008 British Journal of Anaesthesia Terms and Conditions