1. Interleukin-17F increases the secretion of interleukin-8 and the expression of cyclooxygenase 2 in endometriosis 
Tetsuya Hirata, M.D., Ph.D., Yutaka Osuga, M.D., Ph.D., Masashi Takamura, M.D., Ako Saito, M.D., Ph.D., Akiko Hasegawa, M.D., Ph.D., Kaori Koga, M.D., Ph.D., Osamu Yoshino, M.D., Ph.D., Yasushi Hirota, M.D., Ph.D., Miyuki Harada, M.D., Ph.D., Yuji Taketani, M.D., Ph.D. 
Fertility and Sterility 
Volume 96, Issue 1, Pages (July 2011)
DOI: /j.fertnstert
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
(A) Interleukin (IL)-17F secretion from mononuclear cells from endometriotic lesions (EMMCs). The EMMCs secreted IL-17F and the stimulation with phorbol ester (PMA; 50 ng/mL) and ionomycin (1 μg/mL) enhanced the secretion of IL-17F from EMMCs. Concentration of IL-17F in the conditioned medium was measured using a specific ELISA. Values are the means ± SEM of pentaplicate cultures. ∗P<.0001 versus control. The result is representative of three separate experiments using samples from different patients. (B) Immunocytochemistry of IL-17RC in endometriotic stromal cells. Cultured endometriotic stromal cells were immunostained with anti-IL-17RC antibody (i) or goat IgG isotype matched control (ii). Bars = 200 μm.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
(A) Interleukin (IL)-17F stimulates IL-8 secretion from endometriotic stromal cells (ESCs). The ESCs were cultured in 5% fetal bovine serum with different doses of IL-17F for 24 hours. (B) Effect of a mouse anti-IL-17RA antibody or a goat anti-IL-17RC antibody on IL-17F-induced IL-8 secretion by ESCs. ESCs were preincubated in 5% fetal bovine serum medium with or without the antibodies or the control antibodies for 30 minutes and then stimulated with IL-17F (50 ng/mL) for 24 hours. (A, B) Concentration of IL-8 in the conditioned medium was measured using a specific ELISA. Values are the means ± SEM of pentaplicate cultures. Different letters denote significant differences between groups (P<.05). The result is representative of four separate experiments using samples from different patients.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
Effects of interleukin (IL)-17F on tumor necrosis factor α (TNF-α)-mediated IL-8 secretion from endometriotic stromal cells. Endometriotic stromal cells were treated with IL-17F or TNF-α or in combination for 24 hours. The conditioned medium was collected and assayed for IL-8 concentration using a specific ELISA. All values are expressed as the mean ± SEM of pentaplicate cultures. ∗P<.0001 versus each control. The data are representative of four independent experiments.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

5. Figure 4
Effect of interleukin (IL)-17F on the expression of IL-8 (A) and cyclooxygenase 2 (COX2) (B) messenger RNA in endometriotic stromal cells (ESCs). The ESCs were incubated with IL-17F (10 ng/mL) for the indicated duration. Expression of IL-8 and COX2 messenger RNA in ESCs was examined by real-time quantitative polymerase chain reaction (PCR). The data shown are the relative ratio (A, IL-8 to glyceraldehyde-3-phosphate dehydrogenase (GAPDH); B, COX2 to GAPDH) measured by real-time quantitative PCR. Data are the mean ± SEM of five independent experiments using samples from five different patients. Different letters denote significant differences between groups (P<.05).
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions