Presentation is loading. Please wait.

Presentation is loading. Please wait.

Harald Renz, MD, Chaya Brodie, PhD, Katherine Bradley, BS, Donald Y. M

Published byBrigitte Gobeil Modified over 5 years ago

Similar presentations

Presentation on theme: "Harald Renz, MD, Chaya Brodie, PhD, Katherine Bradley, BS, Donald Y. M"— Presentation transcript:

1 Enhancement of IgE production by anti-CD40 antibody in atopic dermatitis 
Harald Renz, MD, Chaya Brodie, PhD, Katherine Bradley, BS, Donald Y.M. Leung, MD, PhD, Erwin W. Gelfand, MD  Journal of Allergy and Clinical Immunology  Volume 93, Issue 3, Pages (March 1994) DOI: /S (94) Copyright © 1994 Mosby, Inc. Terms and Conditions

2 FIG. 1 Induction of IgE production after incubation with anti-CD40 or the combination of anti-CD40 and IL-4. PBMCs from five atopic and five nonatopic donors were cultured with anti-CD40 (0.5 μg/ml), IL-4 (400 U/ml), or tissue culture medium alone for 14 days. IgE production was then analyzed in culture supernatants by ELISA. In each individual the effect of anti-CD40 stimulation was compared with control levels (culture with medium alone) and calculated as percent control (means: atopic donors, 4536 pg/ml; normal donors, 508 pg/ml). Similarly, in IL-4–stimulated cultures the effect of anti-CD40 was compared with cultures treated with IL-4 alone (control) and expressed as percent control (means: atopic donors, 11,144 pg/ml; normal donors, 4046 pg/ml). For each individual, cultures were performed in duplicate. Shown are means ± SD for each experimental group. The asterisk indicates significant (p < 0.05) differences between the groups. Journal of Allergy and Clinical Immunology  , DOI: ( /S (94) ) Copyright © 1994 Mosby, Inc. Terms and Conditions

3 FIG. 2 Effect of anti-CD40 treatment on IgG, IgM, and IgA production. PBMCs from five atopic and five nonatopic donors were cultured with anti-CD40 (0.5 μg/ml) ± IL-4 (400 U/ml) for 14 days, and Ig isotype production was measured in culture supernatants by ELISA. The effect of anti-CD40 was expressed as percent control (cultures with tissue culture medium alone). The baseline levels were: for atopic donors, IgM 868 ng/ml, IgG 926 ng/ml, IgA 95 ng/ml; for normal donors, IgM 716 ng/ml, IgG 672 ng/ml, IgA 104 ng/ml. Shown are means ± SD for each experimental group. NS, Not significant. Journal of Allergy and Clinical Immunology  , DOI: ( /S (94) ) Copyright © 1994 Mosby, Inc. Terms and Conditions

4 FIG. 3 Lymphocyte proliferation induced by anti-IgM, anti-CD40, and IL-4. PBMCs from five atopic patients and five control donors were incubated with anti-IgM (0.5 μg/ml), anti-CD40 (500 ng/ml), and IL-4 (400 U/ml) in different combinations as shown in the figure. The cell cultures were carried out for 72 hours. During the last 6 hours of the culture period tritiated thymidine (TdR) was added (1 μCi/well). In each donor the proliferative response to the reagents was compared with the medium control, and the effect was expressed as percent of the medium control (100%). Expressed are means ± SD from all donors in each group. Mean counts per minute of the medium control were 1345 cpm in the control group and 1947 cpm in the group of atopic patients. Journal of Allergy and Clinical Immunology  , DOI: ( /S (94) ) Copyright © 1994 Mosby, Inc. Terms and Conditions

5 FIG. 4 Analysis of CD40 and CD3 expression on lymphocytes from one normal nonatopic donor (N) and one patient with atopic dermatitis (AD) by double staining. Indicated are MFI and the percentage of CD40-positive cells. Cells were analyzed with a gate for lymphocytes. Journal of Allergy and Clinical Immunology  , DOI: ( /S (94) ) Copyright © 1994 Mosby, Inc. Terms and Conditions

6 FIG. 5 Regulation of CD40 expression by IL-4. PBMCs from two normal donors were incubated with either IL-4 or medium alone for 14 days. At the end of the culture period, lymphocytes were recovered and stained with anti-CD40 as described in the Methods section. Shown are the percentage of CD-40-positive cells and the MFI. The percent of CD-40–positive cells was identical to the percent of CD20-expressing cells (data not shown). Journal of Allergy and Clinical Immunology  , DOI: ( /S (94) ) Copyright © 1994 Mosby, Inc. Terms and Conditions

Download ppt "Harald Renz, MD, Chaya Brodie, PhD, Katherine Bradley, BS, Donald Y. M"

Similar presentations

Proinflammatory cytokine–induced and chemical mediator–induced IL-8 expression in human bronchial epithelial cells through p38 mitogen-activated protein.

Proinflammatory cytokine–induced and chemical mediator–induced IL-8 expression in human bronchial epithelial cells through p38 mitogen-activated protein.
Expression of IL-9 receptor α chain on human germinal center B cells modulates IgE secretion  Lama M. Fawaz, PhD, Ehssan Sharif-Askari, PhD, Oumnia Hajoui,

Expression of IL-9 receptor α chain on human germinal center B cells modulates IgE secretion  Lama M. Fawaz, PhD, Ehssan Sharif-Askari, PhD, Oumnia Hajoui,
Different expression of cytokine and membrane molecules by circulating lymphocytes on acute mental stress in patients with atopic dermatitis in comparison.

Different expression of cytokine and membrane molecules by circulating lymphocytes on acute mental stress in patients with atopic dermatitis in comparison.
Interleukin-4 receptor expression by human B cells: Functional analysis with a human interleukin-4 toxin, DAB389IL-4  Haifa H. Jabara, MSca, Donata Vercelli,

Interleukin-4 receptor expression by human B cells: Functional analysis with a human interleukin-4 toxin, DAB389IL-4  Haifa H. Jabara, MSca, Donata Vercelli,
Interleukin-4 receptor expression by human B cells: Functional analysis with a human interleukin-4 toxin, DAB389IL-4  Haifa H. Jabara, MSca, Donata Vercelli,

Interleukin-4 receptor expression by human B cells: Functional analysis with a human interleukin-4 toxin, DAB389IL-4  Haifa H. Jabara, MSca, Donata Vercelli,
Role of γ/δ T cells in a patient with CD4+ CD3– lymphocytosis, hypereosinophilia, and high levels of IgE  Ilan Bank, MDa,d, Avner Reshef, MDb, Miriam.

Role of γ/δ T cells in a patient with CD4+ CD3– lymphocytosis, hypereosinophilia, and high levels of IgE  Ilan Bank, MDa,d, Avner Reshef, MDb, Miriam.
Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites 

Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites 
The effects of upper respiratory infection on T-cell proliferation and steroid sensitivity of asthmatics  Elcio O. Vianna, MD, PhD, Jay Westcott, PhD,

The effects of upper respiratory infection on T-cell proliferation and steroid sensitivity of asthmatics  Elcio O. Vianna, MD, PhD, Jay Westcott, PhD,
Cell-specific activation profile of extracellular signal-regulated kinase 1/2, Jun N-terminal kinase, and p38 mitogen-activated protein kinases in asthmatic.

Cell-specific activation profile of extracellular signal-regulated kinase 1/2, Jun N-terminal kinase, and p38 mitogen-activated protein kinases in asthmatic.
Decreased serum vitamin D levels in children with asthma are associated with increased corticosteroid use  Daniel A. Searing, MD, Yong Zhang, PhD, James.

Decreased serum vitamin D levels in children with asthma are associated with increased corticosteroid use  Daniel A. Searing, MD, Yong Zhang, PhD, James.
Differential cytokine induction by the human skin–associated autoallergen thioredoxin in sensitized patients with atopic dermatitis and healthy control.

Differential cytokine induction by the human skin–associated autoallergen thioredoxin in sensitized patients with atopic dermatitis and healthy control.
Impaired Responses of Peripheral Blood Mononuclear Cells to Staphylococcal Superantigen in Patients with Severe Atopic Dermatitis: A Role of T Cell Apoptosis 

Impaired Responses of Peripheral Blood Mononuclear Cells to Staphylococcal Superantigen in Patients with Severe Atopic Dermatitis: A Role of T Cell Apoptosis 
Predominance of type 2 cytokine–producing CD4+ and CD8+ cells in patients with atopic dermatitis  Masatoshi Nakazawa, DVMa, Nakako Sugi, MDb, Hiroshi.

Predominance of type 2 cytokine–producing CD4+ and CD8+ cells in patients with atopic dermatitis  Masatoshi Nakazawa, DVMa, Nakako Sugi, MDb, Hiroshi.
Toll-like receptor 7–induced naive human B-cell differentiation and immunoglobulin production  Mark C. Glaum, MD, PhD, Shilpi Narula, MD, Decheng Song,

Toll-like receptor 7–induced naive human B-cell differentiation and immunoglobulin production  Mark C. Glaum, MD, PhD, Shilpi Narula, MD, Decheng Song,
Volume 135, Issue 6, Pages (December 2008)

Volume 135, Issue 6, Pages (December 2008)
Role of IL-9 in the pathophysiology of allergic diseases

Role of IL-9 in the pathophysiology of allergic diseases
CD40-mediated p38 mitogen-activated protein kinase activation is required for immunoglobulin class switch recombination to IgE  Ke Zhang, MD, PhD, Ling.

CD40-mediated p38 mitogen-activated protein kinase activation is required for immunoglobulin class switch recombination to IgE  Ke Zhang, MD, PhD, Ling.
Estrogen enhances immunoglobulin production by human PBMCs

Estrogen enhances immunoglobulin production by human PBMCs
Cell-specific activation profile of extracellular signal-regulated kinase 1/2, Jun N-terminal kinase, and p38 mitogen-activated protein kinases in asthmatic.

Cell-specific activation profile of extracellular signal-regulated kinase 1/2, Jun N-terminal kinase, and p38 mitogen-activated protein kinases in asthmatic.
Differential control of TH1 versus TH2 cell responses by the combination of low-dose steroids with β2-adrenergic agonists  Elena Goleva, PhD, Annegret.

Differential control of TH1 versus TH2 cell responses by the combination of low-dose steroids with β2-adrenergic agonists  Elena Goleva, PhD, Annegret.

Similar presentations

Ads by Google