1. Manipulation of auxin homeostasis leads to ectopic PIN gene expression.
Manipulation of auxin homeostasis leads to ectopic PIN gene expression. (A-H) Inhibition of auxin transport by NPA (50 μmol/l for 24 hours) leads to upregulation of PIN1::PIN1:GFP in epidermis and cortex (B); PIN2::PIN2:HA (D), PIN4::PIN4:GFP (F) and PIN4::GUS (H) in the stele compared with untreated controls (A,C,E,G). (I-L) Treatment for 24 hours with biologically active auxins such as IAA (50 μmol/l, I), 2,4-D (0.1 μmol/l, J) and NAA (10 μmol/l, K) or for 5 days with auxin precursor sirtinol (20 μmol/l, L) leads to an upregulation of PIN4::GUS expression compared with control (G). (M,N) Upregulation of PIN2::GUS expression in root following treatment with 50 μmol/l NAA for 24 hours (N) compared with untreated control (M). (O,P) Treatment for 5 days with auxin precursor sirtinol (20 μmol/l) leads to upregulation of PIN1::PIN1:GFP in epidermis and cortex cells (P) compared with control (O).
Anne Vieten et al. Development 2005;132:
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