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Possible role of cytomegalovirus in the pathogenesis of inflammatory aortic diseases: A preliminary report  Shinji Tanaka, MD, Yasushi Toh, MD, Ryoichi.

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Presentation on theme: "Possible role of cytomegalovirus in the pathogenesis of inflammatory aortic diseases: A preliminary report  Shinji Tanaka, MD, Yasushi Toh, MD, Ryoichi."— Presentation transcript:

1 Possible role of cytomegalovirus in the pathogenesis of inflammatory aortic diseases: A preliminary report  Shinji Tanaka, MD, Yasushi Toh, MD, Ryoichi Mori, MD, Kimihiro Komori, MD, Kenichiro Okadome, MD, Keizo Sugimachi, MD, FACS  Journal of Vascular Surgery  Volume 16, Issue 2, Pages (August 1992) DOI: / (92)90118-R Copyright © 1992 Society for Vascular Surgery and the North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

2 Fig. 1 Design of PCR for detection of CMV DNA. Structure of the IE gene of CMV Towne strain as described by Stenberg et al bp fragment of the fourth exon of IE gene is amplified by primer 1 and primer 2. The nucleotide sequences of these primers and the internal probe for Southern blot analysis are also shown. Journal of Vascular Surgery  , DOI: ( / (92)90118-R) Copyright © 1992 Society for Vascular Surgery and the North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

3 Fig. 2 Histology of inflammatory aneurysm. Thickened fibrotic media and adventitia shows inflammatory infiltrate. (Hematoxylin-eosin stain; original magnification × 8.). Inset shows germinal centers in the media. (original magnification × 35.) Journal of Vascular Surgery  , DOI: ( / (92)90118-R) Copyright © 1992 Society for Vascular Surgery and the North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

4 Fig. 3 Detection of CMV DNA in aortic tissues by PCR. A, Photograph of ethidium-bromide stained gel. B, Southern blot analysis. DNA samples were extracted from inflammatory aneurysms (lanes 1–6), aortic occlusion with inflammation (lanes 7 and 8), atherosclerotic aneurysms (lanes 9–11), autopsy samples (lanes 12–14), and CMV strain AD169 as a positive control (lane 15). No DNA was added as a negative control (lane 16). HpaII-digested pUC18 was used as a size marker (lane M). Positive signals, 230 bp fragment of IE gene, were detected in lanes 1, 2, 4–8, 10, 11, 13, and 15. Journal of Vascular Surgery  , DOI: ( / (92)90118-R) Copyright © 1992 Society for Vascular Surgery and the North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions


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