1. Diagrams representing our five incubation treatments.
Diagrams representing our five incubation treatments. Blue and green colors denote treatments that utilize the city or forest incubation profile, respectively. The temperatures in parentheses are the peak of each thermal spike. One treatment (A) consisted of the city regime looped daily and a thermal spike delivered on day 8 of development. Two treatments (B,C) consisted of the forest regime looped daily and a thermal spike delivered on day 10 of development. Because developmental rate increases with temperature, thermal spikes were delivered 2 days later to eggs incubated at the forest temperatures than those incubated at city temperatures. This ensured that all embryos received the treatment at the same stage of development. Bars show the differential between the peak of the thermal regime and the peak of the thermal spike. Embryo heart rates were recorded for one subset of eggs at the peak temperature of each spike (filled grey circles) and for another subset on the day before and the day after a thermal spike (open circles). Two control treatments (D) consisted of either the forest (continuous line) or city (dashed line) regime repeated daily throughout the entirety of incubation (no thermal spike).
Joshua M. Hall, and Daniel A. Warner J Exp Biol 2018;221:jeb181552
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