1. Ectopic expression of wt-DAO, but not the inactive mutant, promotes senescence.
Ectopic expression of wt-DAO, but not the inactive mutant, promotes senescence. (A) U2OS cells were transfected with pcDNA3-HA containing wt- and R199W-DAO, and the protein expression of DAO was confirmed by immunoblot analysis. (B, C) U2OS cells transfected as in (A) were selected with 800 μg/ml G418 and treated with 2 μM etoposide. After incubation for 7 d, the cells were subjected to SA-β-gal staining (B) and EdU incorporation assay (C). Data are mean ± SD (n = 3 independent experiments). Statistical significance is shown using the t test analysis; *P < 0.05, **P < 0.01.
Taiki Nagano et al. LSA 2019;2:e
© 2019 Nagano et al.