1. (A) yellow cDNA comparison among wild-type and ch mutants
(A) yellow cDNA comparison among wild-type and ch mutants. yellow cDNA of chl11 has a 14-bp deletion in the fourth exon.
(A) yellow cDNA comparison among wild-type and ch mutants. yellow cDNA of chl11 has a 14-bp deletion in the fourth exon. In chk12, the splicing site of intron 3 is mutated (below), and four types cDNA were cloned for yellow. Asterisk indicates the mutation site at the first base of intron 3. Arrows indicate splicing sites of intron 3 in chk12 strain (types 2, 3, 4). Solid boxes, open reading frames; open boxes, untranslated regions; striped boxes, aberrant open reading frames; diagonal lines, introns. (B) ebony cDNA comparison between wild type and so mutant. The large deletion causes a fatal change of the C-terminal amino acid sequence of the Ebony homolog protein. Solid boxes, open reading frames; open boxes untranslated regions; striped box, aberrant open reading frame; diagonal lines, introns; dotted lines, deleted region.
Ryo Futahashi et al. Genetics 2008;180:
Copyright © 2008 by the Genetics Society of America