1. BMP signalling is dispensable for early gastruloid patterning.
BMP signalling is dispensable for early gastruloid patterning. (A) T/Bra::GFP gastruloids stimulated with Chi (48-72 h) AA following a 24 h pulse of either vehicle (top), BMP4 (middle) or DMH1 (bottom; n=12, 13 and 13 at 120 h, respectively), an inhibitor of BMP signalling between 24 and 48 h AA. Normalised fluorescence traces shown per condition (A) with corresponding fluorescence and shape descriptor quantification (B). Inhibition of BMP signalling by DMH1 or activation by BMP4 (24-48 h AA) does not alter the initial patterning of gastruloids; BMP treatment at this time has minimal effect on the subsequent patterning. (C) Gastruloids imaged at 120 h by wide-field microscopy following 24-48 h of vehicle or BMP4 stimulation (pink horizontal box) followed by either vehicle, Chi or BMP4 as indicated (blue horizontal box) between 48 and 72 h AA (n=16 per condition). (D) Stimulation schematic. BMP4 is unable to substitute for Chi in terms of the elongation and patterning of T/Bra, and its sustained expression over time (refer to Fig. S10 for further details and statistical analysis). Scale bar: 200 µm.
David A. Turner et al. Development 2017;144:
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