1. Fig. 1. PolySTAT synthesis and characterization.
PolySTAT synthesis and characterization. (A) The PolySTAT polymer backbone, a linear statistical copolymer of HEMA and NHSMA synthesized via RAFT polymerization, was grafted with the cyclic fibrin-binding peptide Ac-Y(DGl)C(HPr)YGLCYIQGK-Am (19) through NHS ester reaction with the lysine ε-amine. DGl, d-glutamic acid; HPr, hydroxyproline; Ac, acetylated N terminus; Am, amidated C terminus. (B) Two different polymer backbones were used, including a fluorescent FMA-modified p(HEMA-co-NHSMA) for confocal studies. Polymer backbones were grafted with the fibrin-binding peptide for PolySTAT or a nonbinding scrambled peptide for the PolySCRAM control. Polymer molecular weight (MW) and polydispersity index (PDI) were determined using gel permeation chromatography (GPC). Peptides per polymer were calculated using ultraviolet (UV) absorbance. NA, not applicable. (C) PolySTAT integration into fibrin was confirmed using confocal imaging. Pure fibrin clots were made using a solution of Alexa Fluor 546–labeled fibrinogen (red) and thrombin mixed with PBS, fPolySTAT (green), or fPolySCRAM (green). Scale bars, 10 μm.
Leslie W. Chan et al., Sci Transl Med 2015;7:277ra29
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