1. Fig. 6. Apical polarity of primitive endoderm cells on surface of embryoid bodies.Embryoid bodies were analyzed by immunofluorescence microscopy for GATA4 (primitive endoderm marker), Dab2 (primitive endoderm marker), and megalin (primitive endoderm apical polarity marker).
Apical polarity of primitive endoderm cells on surface of embryoid bodies.Embryoid bodies were analyzed by immunofluorescence microscopy for GATA4 (primitive endoderm marker), Dab2 (primitive endoderm marker), and megalin (primitive endoderm apical polarity marker). (A) A representative embryoid body derived from wildtype ES cells. Dab2 (cytoplasmic) and GATA4 (nuclear) mark the surface layer of primitive endoderm cells. Megalin is restricted to the apical membrane; (B) A 4-day-old embryoid body derived from E-cadherin null ES cells is shown for example. Dab2 staining marks both internal and surface primitive endoderm cells. Megalin staining indicates that primitive endoderm cells located inside the spheroid shows no polarity, and the cells located on surface display various degree of apical polarization (degree of megalin apical restriction), from less (indicated by *) to more (indicated by **) localization to apical surface. (C) A more mature (7-day-old) embryoid body derived from E-cadherin null ES cells shows strong apical polarity of megalin of the primitive endoderm cells on surface. Scale bars: 10 µm (A,B), 5 µm (C).
Robert Moore et al. Biology Open 2014;3:
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