1. Immunoexpression of hepatocyte growth factor and c-Met receptor in the eutopic endometrium predicts the activity of ectopic endometrium 
Khaleque Newaz Khan, M.D., Ph.D., Hideaki Masuzaki, M.D., Akira Fujishita, M.D., Michio Kitajima, M.D., Ichiro Sekine, M.D., Tadayuki Ishimaru, M.D. 
Fertility and Sterility 
Volume 79, Issue 1, Pages (January 2003)
DOI: /S (02)

2. FIGURE 1
Immunohistochemical staining of HGF and its receptor, c-Met, in the eutopic endometrium of women with or without endometriosis. The tissue specimens shown in panels a, b, and c were taken from women with endometriosis, and the tissue specimens shown in panels d, e, and f were taken from women without endometriosis. Both of them were obtained from the secretory phase of the menstrual cycle. (a and d), Hematoxylin and eosin staining. (b and e), Immunohistochemical staining of HGF. (c and f), Immunohistochemical staining of c-Met. Immunohistochemical staining of HGF and c-Met in the eutopic endometrium of women with endometriosis appeared to be stronger than in that of women without endometriosis. Final magnification was adjusted at ×50 (a–f) using a light microscope connected to a camera (Olympus-VANOX, model-AHBS, Tokyo, Japan).
Khan. HGF/c-Met immunoexpression in endometriosis. Fertil Steril 2003.
Fertility and Sterility  , DOI: ( /S (02) )

3. FIGURE 2
Q-H scores of HGF, c-Met, VEGF, and PCNA in the epithelium and stroma and MVC in the stroma of women with and without endometriosis. (A), Q-H scores in all women with or without endometriosis. An asterisk (*) denotes a significant difference from women without endometriosis. (B), Q-H scores in women with endometriosis according to r-ASRM stage. (C), Q-H scores distributed according to the r-ASRM morphologic classification. An asterisk denotes a significant difference from either black or white lesion. The results are expressed as mean ± SEM, and the details are described in the Results section. E, epithelium; S, stroma.
Khan. HGF/c-Met immunoexpression in endometriosis. Fertil Steril 2003.
Fertility and Sterility  , DOI: ( /S (02) )

4. FIGURE 3
Immunohistochemical staining of HGF and c-Met in different pigmented lesions of the ectopic endometrium. All tissue specimens were obtained from the secretory phase of the menstrual cycle. (a and d), Hematoxilin and eosin staining of red and black lesions, respectively. (b and e), Immunohistochemical staining of HGF in red and black lesions, respectively. (c and f), Immunohistochemical staining of c-Met in red and black lesions, respectively. Final magnification was adjusted at ×50 (a–f) using a light microscope connected to a camera (Olympus-VANOX, model-AHBS).
Khan. HGF/c-Met immunoexpression in endometriosis. Fertil Steril 2003.
Fertility and Sterility  , DOI: ( /S (02) )

5. FIGURE 4
Immunoreactions of the eutopic and ectopic endometrium used as negative controls (a–d), immunoexpressions of HGF and c-Met in the pelvic mesothelium (e and f, respectively) and immunoreactions of HGF and c-Met in the placental tissue used as a positive control (g and h, respectively). Nonimmune mouse IgG1 antibody was used for negative control specimens instead of first antibody. (a), Eutopic endometrium of control woman. (b), Eutopic endometrium of women with endometriosis. (c), Red lesion. (d), Black lesion. Tissue specimens of a–d were the same as in Figures 1 and 3. Final magnification was adjusted at ×20 (a–h) using light microscope connected to a camera (Olympus-VANOX, model-AHBS).
Khan. HGF/c-Met immunoexpression in endometriosis. Fertil Steril 2003.
Fertility and Sterility  , DOI: ( /S (02) )

6. FIGURE 5
Q-H scores of HGF, c-Met, VEGF, and PCNA in the epithelium and stroma and the MVC in the stroma of the eutopic and ectopic endometrium of women with and without endometriosis. (A), Q-H scores in the eutopic endometrium of 10 control women according to menstrual cycle. (B), Q-H scores in the eutopic endometrium of 15 women with endometriosis according to menstrual cycle. (C), Overall activity as represented by the combined Q-H scores in the epithelium and stroma of the eutopic and ectopic endometrium of women with and without endometriosis. An asterisk (*) denotes a significant difference from the eutopic endometrium of control women or from other peritoneal lesions. The results are expressed as mean ± SEM, and the details are described in the Results section. E, epithelium; S, stroma.
Khan. HGF/c-Met immunoexpression in endometriosis. Fertil Steril 2003.
Fertility and Sterility  , DOI: ( /S (02) )