1. Induction of apoptosis by nelfinavir and bortezomib
Supplemental Figure 4
Induction of apoptosis by nelfinavir and bortezomib
Amo-1 cells were treated with nelfinavir (nelfi) mM alone or in combination with bortezomib (bzb) 20 nM and the surface expression of annexin V and 7-AAD were quantified by flow cytometry. The numbers in the individual quadrants represent the relative fraction of cells in each quadrant as % of total cells.
bzb
untreated
7.8
18.5
2.0
6.0
22.4
15.7
nelfi 10µM
7.0
25.8
4.8
nelfi 20 µM
14.0
34.5
3.1
bzb+nelfi10µM
14.6
27.5
3.0
bzb+nelfi 20µM
17.1
32.0
3.8
7-AAD
annexin V