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Dominance of Nonmalignant T-Cell Clones and Distortion of the TCR Repertoire in the Peripheral Blood of Patients with Cutaneous CD30+ Lymphoproliferative.

Published byCornelia Murphy Modified over 5 years ago

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Presentation on theme: "Dominance of Nonmalignant T-Cell Clones and Distortion of the TCR Repertoire in the Peripheral Blood of Patients with Cutaneous CD30+ Lymphoproliferative."— Presentation transcript:

1 Dominance of Nonmalignant T-Cell Clones and Distortion of the TCR Repertoire in the Peripheral Blood of Patients with Cutaneous CD30+ Lymphoproliferative Disorders  Daniel Humme, Ansgar Lukowsky, Matthias Steinhoff, Marc Beyer, Peter Walden, Wolfram Sterry, Chalid Assaf  Journal of Investigative Dermatology  Volume 129, Issue 1, Pages (January 2009) DOI: /jid Copyright © 2009 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 Serial dilution of the clonal T-cell line MyLa in pooled PBMCs of 10 healthy donors. TCR-γ PCR assays of tubes A (left side) and B (right side). Dilution steps were performed at 100, 50, 25, 10, 5, 2, and 1% clonal cells, showing a sensitivity of 2–5% (MyLa cell line reveals a PCR product of 196 base pairs in tube A and 182 base pairs in tube B); x axis, length of PCR products in base pairs (bp). Journal of Investigative Dermatology  , 89-98DOI: ( /jid ) Copyright © 2009 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 Comparison of clonal T-cell populations of lesional skin and peripheral blood of patient 9 (tube A). (Left side) Skin specimens were obtained in (a), (b), and (c) revealing an identical size of 145bp. (Right side) Clonality of three separate blood specimens each with a fragment size of 159bp. Specimens were obtained in (a), (b), and (c). Journal of Investigative Dermatology  , 89-98DOI: ( /jid ) Copyright © 2009 The Society for Investigative Dermatology, Inc Terms and Conditions

4 Figure 3 Comparison of clonal T-cell populations of lesional skin and peripheral blood. Identical clonal (biallelic) rearrangement of TCR -γ in two separate skin biopsies of the patient 15 with LyP (a, b). No clonal rearrangement (polyclonal distribution) in two separate blood samples (c, d) profiles. (Left side) Profiles of tube A, (right side) profiles of tube B. Journal of Investigative Dermatology  , 89-98DOI: ( /jid ) Copyright © 2009 The Society for Investigative Dermatology, Inc Terms and Conditions

5 Figure 4 Correlation of immunoscope analysis with T-cell receptor gene rearrangement. Immunoscope analysis (a) and corresponding GeneScan profiles, tubes A and B (b). Patient 8 (left side) with LyP who developed an associated lymphoma (Mf) demonstrated a clear restriction in the T-cell receptor repertoire and a monoclonal TCR-γ rearrangement in the peripheral blood. Patient 1 (middle) with LyP demonstrated an oligoclonal TCR-γ rearrangement in association with an altered CDR3 length distribution whereas patient 15 (right side) with LyP presented a polyclonal GeneScan profile and a nearly Gaussian TCR-β CDR3 length distribution in the peripheral blood. Journal of Investigative Dermatology  , 89-98DOI: ( /jid ) Copyright © 2009 The Society for Investigative Dermatology, Inc Terms and Conditions

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