1. A simple comet assay for archived sperm correlates DNA fragmentation to reduced hyperactivation and penetration of zona-free hamster oocytes 
Philip J Chan, Ph.D., Johannah U Corselli, Ph.D., William C Patton, M.D., John D Jacobson, M.D., Steven R Chana, Alan King, M.D. 
Fertility and Sterility 
Volume 75, Issue 1, Pages (January 2001)
DOI: /S (00)

2. Figure 1
Modified alkaline comet assay of dried sperm smear. The fixed sperm were stained with acridine orange, layered over with a mini-agarose gel, lysed, processed by using electrophoresis, and viewed under fluorescent microscopy. The images were captured by holding an inexpensive computer videocamera to the eyepiece of the microscope; a shareware imaging program was used to convert the images to gray scale for pixel analysis. (A), Electrophoresis equipment, with paper stickers on slides; (B), sperm nuclei, with normal intact DNA forming halos; (C), necrosis, with diffuse comet tails; (D), apoptosis, with fan-like distinct tails. Lighter DNA density at the center of each sperm nucleus represents increased fragmentation of DNA.
Chan. Comet assay of archived sperm. Fertil Steril 2001.
Fertility and Sterility  , DOI: ( /S (00) )