1. Transcriptomic profiling of host cells during ZIKV infection.
Transcriptomic profiling of host cells during ZIKV infection. Primary human MNCs and MDMs (2 × 106 cells per infection) were infected with ZIKV at an MOI of 10, harvested at 24 and 72 hpi for transcriptomic analysis by RNA-seq, and then compared to mock-infected controls. (A) Venn diagram illustrating the proportion of upregulated signaling pathways identified by IPA in ZIKV-infected MDMs. Upregulation intensities of the 27 common canonical pathways are shown in a heat map. Asterisks within the boxes represent the calculated P values associated with each identified pathway compared to the mock-infected samples. (B) The five most upregulated genes within the top three signaling pathways (interferon pathway, multiple sclerosis pathway, and cross talk between moDCs and NK cells) at 24 and 72 hpi are shown. Data presented were obtained from a total of five donors.
Fok-Moon Lum et al. mSphere 2018; doi: /mSphereDirect