1. Actin network polarisation.
Actin network polarisation. (A) Cells were plated on discoidal micropatterns (green). Cell actin architecture was initially mainly composed of an isotropic branched meshwork all along cell periphery (green crosses). A bar perpendicular to disc border was added with laser patterning (red). Cells rapidly formed stress fibers connecting the disc and the bar tip (red bars). Pre-existing structures are drawn in green and new ones are in red. (B) During this transformation, actin network architecture remodeling was monitored with Lifeact–GFP. Stress fibers were clearly visible after 30 minutes. They then get thicker and longer as the cells extended along the bar. Scale bar: 10 μm.
Timothée Vignaud et al. J Cell Sci 2012;125:
© 2012.